PUBLICATION
Single-cell resolution of MET- and EMT-like programs in osteoblasts during zebrafish fin regeneration
- Authors
- Tang, W.J., Watson, C.J., Olmstead, T., Allan, C.H., Kwon, R.Y.
- ID
- ZDB-PUB-220217-1
- Date
- 2022
- Source
- iScience 25: 103784 (Journal)
- Registered Authors
- Kwon, Ronald, Watson, Claire
- Keywords
- Biological sciences, Developmental biology, Omics, Transcriptomics
- Datasets
- GEO:GSE192498
- MeSH Terms
- none
- PubMed
- 35169687 Full text @ iScience
Citation
Tang, W.J., Watson, C.J., Olmstead, T., Allan, C.H., Kwon, R.Y. (2022) Single-cell resolution of MET- and EMT-like programs in osteoblasts during zebrafish fin regeneration. iScience. 25:103784.
Abstract
Zebrafish regenerate fin rays following amputation through epimorphic regeneration, a process that has been proposed to involve the epithelial-to-mesenchymal transition (EMT). We performed single-cell RNA sequencing (scRNA-seq) to elucidate osteoblastic transcriptional programs during zebrafish caudal fin regeneration. We show that osteoprogenitors are enriched with components associated with EMT and its reverse, mesenchymal-to-epithelial transition (MET), and provide evidence that the EMT markers cdh11 and twist2 are co-expressed in dedifferentiating cells at the amputation stump at 1 dpa, and in differentiating osteoblastic cells in the regenerate, the latter of which are enriched in EMT signatures. We also show that esrp1, a regulator of alternative splicing in epithelial cells that is associated with MET, is expressed in a subset of osteoprogenitors during outgrowth. This study provides a single cell resource for the study of osteoblastic cells during zebrafish fin regeneration, and supports the contribution of MET- and EMT-associated components to this process.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping