PUBLICATION
Computational simulations of the 4D micro-circulatory network in zebrafish tail amputation and regeneration
- Authors
- Roustaei, M., In Baek, K., Wang, Z., Cavallero, S., Satta, S., Lai, A., O'Donnell, R., Vedula, V., Ding, Y., Marsden, A.L., Hsiai, T.K.
- ID
- ZDB-PUB-220216-34
- Date
- 2022
- Source
- Journal of the Royal Society, Interface 19: 20210898 (Journal)
- Registered Authors
- Keywords
- haemodynamic shear stress, microvascular injury and regeneration, non-Newtonian blood flow, vascular remodelling
- MeSH Terms
-
- Amputation, Surgical
- Animals
- Blood Flow Velocity
- Hemodynamics
- Mechanotransduction, Cellular*
- Shear Strength
- Stress, Mechanical
- Zebrafish*
- PubMed
- 35167770 Full text @ J. R. Soc. Interface
Citation
Roustaei, M., In Baek, K., Wang, Z., Cavallero, S., Satta, S., Lai, A., O'Donnell, R., Vedula, V., Ding, Y., Marsden, A.L., Hsiai, T.K. (2022) Computational simulations of the 4D micro-circulatory network in zebrafish tail amputation and regeneration. Journal of the Royal Society, Interface. 19:20210898.
Abstract
Wall shear stress (WSS) contributes to the mechanotransduction underlying microvascular development and regeneration. Using computational fluid dynamics, we elucidated the interplay between WSS and vascular remodelling in a zebrafish model of tail amputation and regeneration. The transgenic Tg (fli1:eGFP; Gata1:ds-red) zebrafish line was used to track the three-dimensional fluorescently labelled vascular endothelium for post-image segmentation and reconstruction of the fluid domain. Particle image velocimetry was used to validate the blood flow. Following amputation to the dorsal aorta and posterior cardinal vein (PCV), vasoconstriction developed in the dorsal longitudinal anastomotic vessel (DLAV) along with increased WSS in the proximal segmental vessels (SVs) from amputation. Angiogenesis ensued at the tips of the amputated DLAV and PCV where WSS was minimal. At 2 days post amputation (dpa), vasodilation occurred in a pair of SVs proximal to amputation, followed by increased blood flow and WSS; however, in the SVs distal to amputation, WSS normalized to the baseline. At 3 dpa, the blood flow increased in the arterial SV proximal to amputation and through anastomosis with DLAV formed a loop with PCV. Thus, our in silico modelling revealed the interplay between WSS and microvascular adaptation to changes in WSS and blood flow to restore microcirculation following tail amputation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping