PUBLICATION
Impacts of diethylhexyl phthalate and overfeeding on physical fitness and lipid mobilization of Danio rerio (zebrafish)
- Authors
- Buerger, A.N., Parente, C.E., Harris, J.P., Watts, E.G., Wormington, A.M., Bisesi, J.H.
- ID
- ZDB-PUB-220126-31
- Date
- 2022
- Source
- Chemosphere 295: 133703 (Journal)
- Registered Authors
- Keywords
- Critical swimming speed, Diethylhexyl phthalate, Lipid mobilization, Obesity, Physical fitness, Zebrafish
- MeSH Terms
-
- Animals
- Diethylhexyl Phthalate*/metabolism
- Diethylhexyl Phthalate*/toxicity
- Lipid Mobilization
- Physical Fitness
- Plasticizers/metabolism
- Plasticizers/toxicity
- Zebrafish*/metabolism
- PubMed
- 35066078 Full text @ Chemosphere
Citation
Buerger, A.N., Parente, C.E., Harris, J.P., Watts, E.G., Wormington, A.M., Bisesi, J.H. (2022) Impacts of diethylhexyl phthalate and overfeeding on physical fitness and lipid mobilization of Danio rerio (zebrafish). Chemosphere. 295:133703.
Abstract
As the prevalence of obesity has steadily increased on a global scale, research has shifted to explore potential contributors to this pandemic beyond overeating and lack of exercise. Environmental chemical contaminants, known as obesogens, alter metabolic processes and exacerbate the obese phenotype. Diethylhexyl phthalate (DEHP) is a common chemical plasticizer found in medical supplies, food packaging, and polyvinyl materials, and has been identified as a probable obesogen. This study investigated the hypothesis that co-exposure to DEHP and overfeeding would result in decreased lipid mobilization and physical fitness in Danio rerio (zebrafish). Four treatment groups were randomly assigned: Regular Fed (control, 10 mg/fish/day with 0 mg/kg DEHP), Overfed (20 mg/fish/day with 0 mg/kg DEHP), Regular Fed + DEHP (10 mg/fish/day with 3 mg/kg DEHP), Overfed + DEHP (20 mg/fish/day with 3 mg/kg DEHP). After 24 weeks, swim tunnel assays were conducted on half of the zebrafish from each treatment to measure critical swimming speeds (Ucrit); the other fish were euthanized without swimming. Body mass index (BMI) was measured, and tissues were collected for blood lipid characterization and gene expression analyses. Co-exposure to DEHP and overfeeding decreased swim performance as measured by Ucrit. While no differences in blood lipids were observed with DEHP exposure, differential expression of genes related to lipid metabolism and utilization in the gastrointestinal and liver tissue suggests alterations in metabolism and lipid packaging, which may impact utilization and ability to mobilize lipid reserves during physical activity following chronic exposures.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping