PUBLICATION

Power-effective scanning with AODs for 3D optogenic applications

Authors
Ricci, P., Marchetti, M., Sorelli, M., Turrini, L., Resta, F., Gavryusev, V., de Vito, G., Sancataldo, G., Vanzi, F., Silvestri, L., Pavone, F.S.
ID
ZDB-PUB-220111-5
Date
2022
Source
Journal of biophotonics   15(4): e202100256 (Journal)
Registered Authors
Turrini, Lapo, Vanzi, Francesco
Keywords
none
MeSH Terms
  • Optogenetics
  • Brain/diagnostic imaging
  • Animals
  • Neurons*
  • Zebrafish*
  • Photic Stimulation/methods
(all 6)
PubMed
35000289 Full text @ J. Biophotonics
Abstract
Two-photon (2P) excitation is a cornerstone approach widely employed in neuroscience microscopy for deep optical access and sub-micrometric resolution light-targeting into the brain. However, besides structural and functional imaging, 2P optogenetic stimulations are less routinary, especially in 3D. This is because of the adopted scanning systems, often feebly effective, slow and mechanically constricted. Faster illumination can be achieved through acousto-optic deflectors (AODs) although their applicability to large volumes excitation has been limited by large efficiency drop along the optical axis. Here, we present a new AOD-based scheme for 2P 3D-scanning that improves the power delivery between different illumination planes. We applied this approach to photostimulate an optogenetic actuator in zebrafish larvae, demonstrating the method efficiency observing increased activity responses and uniform activation probabilities from neuronal clusters addressed in the volume. This novel driving scheme can open to new AOD applications in neuroscience, allowing more effective 3D-interrogation in large neuronal networks. This article is protected by copyright. All rights reserved.
Genes / Markers
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Expression
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b4
    		Insertion
    jf5TgTransgenic Insertion
      zf3659TgTransgenic Insertion
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        Fish
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        Engineered Foreign Genes
        Marker Marker Type Name
        GCaMPEFGGCaMP
        ReaChREFGReaChR
        TagRFPEFGTagRFP
        1 - 3 of 3
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        Citation
        Ricci, P., Marchetti, M., Sorelli, M., Turrini, L., Resta, F., Gavryusev, V., de Vito, G., Sancataldo, G., Vanzi, F., Silvestri, L., Pavone, F.S. (2022) Power-effective scanning with AODs for 3D optogenic applications. Journal of biophotonics. 15(4):e202100256.