PUBLICATION
Pidotimod increases inflammation in wounded zebrafish embryos
- Authors
- Ding, L., Luo, K., Feng, C.G., Oehlers, S.H.
- ID
- ZDB-PUB-211221-21
- Date
- 2021
- Source
- Fish & shellfish immunology 120: 429-433 (Journal)
- Registered Authors
- Luo, Kaiming, Oehlers, Stefan
- Keywords
- Inflammation, Pidotimod, Tail wound, Toxicity, Zebrafish
- MeSH Terms
-
- Animals
- Inflammation*/chemically induced
- Larva
- Pyrrolidonecarboxylic Acid/adverse effects
- Pyrrolidonecarboxylic Acid/analogs & derivatives*
- Thiazolidines/adverse effects*
- Zebrafish*
- PubMed
- 34922016 Full text @ Fish Shellfish Immunol.
Citation
Ding, L., Luo, K., Feng, C.G., Oehlers, S.H. (2021) Pidotimod increases inflammation in wounded zebrafish embryos. Fish & shellfish immunology. 120:429-433.
Abstract
Pidotimod (PDT) is a synthetic dipeptide molecule which can improve immune responses in mice and humans, protecting hosts from infection. However, the exact mechanism of protection remains ill-defined. The effect of pidotimod has not yet been investigated in the inflammatory response of zebrafish. In this study, we used tail wound and infection models of zebrafish to study the effect of PDT on inflammation. We found that zebrafish larvae were sensitive to PDT immersion causing toxicity at doses above 50 μg/mL. The tail wound assay showed that PDT increased the recruitment of neutrophils and macrophages to the wound site and promoted the transcription of the pro-inflammatory cytokine il1b. However, we did not observe protection of uropathogenic Escherichia coli or Mycobacterium marinum infected zebrafish larvae following PDT treatment. This study provides a new platform for PDT research, which is worthy of further research to identify further effects of PDT therapy.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping