PUBLICATION
High-Resolution Histology for Craniofacial Studies on Zebrafish and Other Teleost Models
- Authors
- Huysseune, A., Soenens, M., Sire, J.Y., Witten, P.E.
- ID
- ZDB-PUB-211217-8
- Date
- 2022
- Source
- Methods in molecular biology (Clifton, N.J.) 2403: 249-262 (Other)
- Registered Authors
- Huysseune, Ann, Sire, Jean-Yves, Witten, P. Eckhard
- Keywords
- Epon 812, Epoxy embedding, Paraffin, Plastic section histology, Semithin sections
- MeSH Terms
-
- Animals
- Bone and Bones
- Epoxy Resins
- Histological Techniques*
- Microscopy, Electron, Transmission
- Skull*
- Staining and Labeling
- Zebrafish*/genetics
- PubMed
- 34913128 Full text @ Meth. Mol. Biol.
Citation
Huysseune, A., Soenens, M., Sire, J.Y., Witten, P.E. (2022) High-Resolution Histology for Craniofacial Studies on Zebrafish and Other Teleost Models. Methods in molecular biology (Clifton, N.J.). 2403:249-262.
Abstract
In the era of molecular biology, identification of cells and even tissues mostly relies on the presence of fluorescent tags, or of "marker gene" expression. We list a number of caveats and present a protocol for embedding, sectioning, and staining semithin plastic sections. The method is neither new nor innovative, but is meant to revive skills that tend to get lost.This easy-to-use and inexpensive protocol (1) yields high-resolution images in transmitted and polarized light, (2) can be utilized simultaneously for transmission electron microscopy, and (3) is applicable to any type of material (wild type, morphants, mutants, transgenic, or pharmacologically treated animals as well as all of their controls), provided the sample size is kept under a limit. Thus, we hope to encourage researchers to use microanatomy and histology to complement molecular studies investigating, e.g., gene function.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping