PUBLICATION
FGF binding protein 3 is required for spinal cord motor neuron development and regeneration in zebrafish
- Authors
- Xu, G., Huang, Z., Sheng, J., Gao, X., Xin, W., Garcia, J.Q., Wei, G., Liu, D., Gong, J.
- ID
- ZDB-PUB-211214-47
- Date
- 2021
- Source
- Experimental neurology 348: 113944 (Journal)
- Registered Authors
- Gong, Jie, Liu, Dong
- Keywords
- Fgfbp3, Morphogenesis, Motor neurons, Regeneration, Zebrafish
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Animals, Genetically Modified
- Carrier Proteins/antagonists & inhibitors
- Carrier Proteins/biosynthesis*
- Carrier Proteins/genetics*
- Gene Knockout Techniques/methods
- Motor Neurons/metabolism*
- Nerve Regeneration/physiology*
- Neurogenesis/physiology*
- Reflex, Startle/physiology
- Spinal Cord/growth & development
- Spinal Cord/metabolism*
- Swimming/physiology
- Zebrafish
- PubMed
- 34896115 Full text @ Exp. Neurol.
Citation
Xu, G., Huang, Z., Sheng, J., Gao, X., Xin, W., Garcia, J.Q., Wei, G., Liu, D., Gong, J. (2021) FGF binding protein 3 is required for spinal cord motor neuron development and regeneration in zebrafish. Experimental neurology. 348:113944.
Abstract
Fibroblast growth factor binding protein 3 (Fgfbp3) have been known to be crucial for the process of neural proliferation, differentiation, migration, and adhesion. However, the specific role and the molecular mechanisms of fgfbp3 in regulating the development of motor neurons remain unclear. In this study, we have investigated the function of fgfbp3 in morphogenesis and regeneration of motor neuron in zebrafish. Firstly, we found that fgfbp3 was localized in the motor neurons and loss of fgfbp3 caused the significant decrease of the length and branching number of the motor neuron axons, which could be partially rescued by fgfbp3 mRNA injection. Moreover, the fgfbp3 knockdown (KD) embryos demonstrated similar defects of motor neurons as identified in fgfbp3 knockout (KO) embryos. Furthermore, we revealed that the locomotion and startle response of fgfbp3 KO embryos were significantly restricted, which were partially rescued by the fgfbp3 overexpression. In addition, fgfbp3 KO remarkably compromised axonal regeneration of motor neurons after injury. Lastly, the malformation of motor neurons in fgfbp3 KO embryos was rescued by overexpressing drd1b or neurod6a, respectively, which were screened by transcriptome sequencing. Taken together, our results provide strong cellular and molecular evidence that fgfbp3 is crucial for the axonal morphogenesis and regeneration of motor neurons in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping