PUBLICATION

Mechanisms underlying microglial colonization of developing neural retina in zebrafish

Authors
Ranawat, N., Masai, I.
ID
ZDB-PUB-211211-1
Date
2021
Source
eLIFE   10: (Journal)
Registered Authors
Masai, Ichiro, Ranawat, Nishtha
Keywords
blood vessel, developmental biology, microglia, neurogenesis, neuroscience, retina, zebrafish
MeSH Terms
  • Retinal Vessels
  • Animals
  • Zebrafish/growth & development*
  • Stem Cells/cytology
  • Cell Movement
  • Microglia/cytology
  • Microglia/physiology*
  • Neurogenesis/physiology
  • Retina/cytology
  • Retina/growth & development*
(all 10)
PubMed
34872632 Full text @ Elife
Abstract
Microglia are brain-resident macrophages that function as the first line of defense in brain. Embryonic microglial precursors originate in peripheral mesoderm and migrate into the brain during development. However, the mechanism by which they colonize the brain is incompletely understood. The retina is one of the first brain regions to accommodate microglia. In zebrafish, embryonic microglial precursors use intraocular hyaloid blood vessels as a pathway to migrate into the optic cup via the choroid fissure. Once retinal progenitor cells exit the cell cycle, microglial precursors associated with hyaloid blood vessels start to infiltrate the retina preferentially through neurogenic regions, suggesting that colonization of retinal tissue depends upon the neurogenic state. Along with blood vessels and retinal neurogenesis, IL34 also participates in microglial precursor colonization of the retina. Altogether, CSF receptor signaling, blood vessels, and neuronal differentiation function as cues to create an essential path for microglial migration into developing retina.
Genes / Markers
Figures
Figure Gallery (6 images)
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Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
a9
    		Complex
    hkz11
      		Small Deletion
      kca4TgTransgenic Insertion
        nkuasgfp1aTgTransgenic Insertion
          oki011TgTransgenic Insertion
            oki053TgTransgenic Insertion
              oki058TgTransgenic Insertion
                oki065TgTransgenic Insertion
                  rw021TgTransgenic Insertion
                    rw440
                      		Point Mutation
                      1 - 10 of 12
                      Show
                      Human Disease / Model
                      No data available
                      Sequence Targeting Reagents
                      Target Reagent Reagent Type
                      atoh7MO1-atoh7MRPHLNO
                      cxcl12aMO1-cxcl12aMRPHLNO
                      spi1bMO1-spi1bMRPHLNO
                      tnnt2aMO1-tnnt2aMRPHLNO
                      tp53MO4-tp53MRPHLNO
                      1 - 5 of 5
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                      Fish
                      No data available
                      Antibodies
                      Orthology
                      No data available
                      Engineered Foreign Genes
                      Marker Marker Type Name
                      EGFPEFGEGFP
                      GAL4EFGGAL4
                      GFPEFGGFP
                      mCherryEFGmCherry
                      TomatoEFGTomato
                      1 - 5 of 5
                      Show
                      Mapping
                      No data available
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                      Citation
                      Ranawat, N., Masai, I. (2021) Mechanisms underlying microglial colonization of developing neural retina in zebrafish. eLIFE. 10:.