PUBLICATION
Screening of Potential Anti-Thrombotic Ingredients from Salvia miltiorrhiza in Zebrafish and by Molecular Docking
- Authors
- Tang, H., Qin, N., Rao, C., Zhu, J., Wang, H., Hu, G.
- ID
- ZDB-PUB-211129-68
- Date
- 2021
- Source
- Molecules 26(22): (Journal)
- Registered Authors
- Keywords
- anti-thrombotic effects, lithospermic acid, luteolin-7-O-β-d-glucoside, molecular docking, rosmarinic acid, salvianolic acid B, zebrafish
- MeSH Terms
-
- Animals
- Disease Models, Animal
- Drug Evaluation, Preclinical
- Drugs, Chinese Herbal/chemistry
- Drugs, Chinese Herbal/isolation & purification
- Drugs, Chinese Herbal/pharmacology*
- Fibrinolytic Agents/chemistry
- Fibrinolytic Agents/isolation & purification
- Fibrinolytic Agents/pharmacology*
- Humans
- Medicine, Chinese Traditional
- Molecular Docking Simulation
- Molecular Structure
- Plants, Medicinal/chemistry
- Salvia miltiorrhiza/chemistry*
- Thrombosis/blood
- Thrombosis/drug therapy
- Zebrafish
- PubMed
- 34833900 Full text @ Molecules
Citation
Tang, H., Qin, N., Rao, C., Zhu, J., Wang, H., Hu, G. (2021) Screening of Potential Anti-Thrombotic Ingredients from Salvia miltiorrhiza in Zebrafish and by Molecular Docking. Molecules. 26(22):.
Abstract
Background Danshen (DS), the dry root of Salvia miltiorrhiza Bge., has been used in traditional Chinese medicine (TCM) for many years to promote blood circulation and to inhibit thrombosis. However, the active ingredients responsible for the anti-thrombotic effect and the underlying mechanisms are yet to be fully elucidated.
Methods Molecular docking was used to predict the active ingredients in DS and their potential targets by calculating the scores of docking between DS ingredients and thrombosis-related proteins. Then, a chemical-induced zebrafish thrombosis model was applied to confirm their anti-thrombotic effects.
Result The molecular docking results indicated that compared to the control ligand, higher docking scores were observed for several compounds in DS, among which salvianolic acid B (SAB), lithospermic acid (LA), rosmarinic acid (MA), and luteolin-7-O-β-d-glucoside (LG) could attenuate zebrafish caudal vein thrombosis and recover the decrease in heart red blood cells (RBCs) in a dose-dependent manner.
Conclusions Our study showed that it is possible to screen the potential active components in natural products by combining the molecular docking method and zebrafish in vivo model.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping