PUBLICATION
Establishment of a rotavirus-infected zebrafish model and its application in drug screening
- Authors
- Song, L., Zhu, X., Zhou, Y., Feng, Y., Dai, G., Chen, J., Chen, Y., Li, F., Zhao, W.
- ID
- ZDB-PUB-211116-49
- Date
- 2021
- Source
- Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 145: 112398 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Survival Rate
- Antiviral Agents/pharmacology
- Disease Models, Animal
- Drug Evaluation, Preclinical
- Diarrhea/virology*
- Rotavirus/isolation & purification*
- Larva
- Rotavirus Infections/virology*
- Zebrafish
- Animals
- PubMed
- 34781142 Full text @ Biomed. Pharmacother.
Citation
Song, L., Zhu, X., Zhou, Y., Feng, Y., Dai, G., Chen, J., Chen, Y., Li, F., Zhao, W. (2021) Establishment of a rotavirus-infected zebrafish model and its application in drug screening. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. 145:112398.
Abstract
Rotavirus (RV) is one of the main pathogens that induce infantile diarrhea and by now no effective drugs are available for RV-induced infantile diarrhea. Thus the development of novel models is of vital importance for the pathological research of RV-induced infantile diarrhea, as well as the progress of the associated treatment strategy. Here we introduced for the first time that RV-Wa strain and RV-SA-11 strain could infect 5 dpf(day post fertilization) and 28 dpf larvae, to induce infantile diarrhea model that was highly consistent with the clinical infection of infants. RV infection significantly changed the signs, survival rate and inflammation of larvae. Some important indicators, including the levels of RV antigen VP4 and VP6, the in vivo RV tracking, and the RV particles were also analyzed, which collectively demonstrated that the model was successfully established. More importantly, we also determined the potentials of the proposed RV-infected zebrafish model for anti-viral drug assessment. In conclusion, we established a RV-infected zebrafish model with formulated relevant indicators both larvae and adult fish, which might be served as a high throughput platform for antiviral drug screening.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping