PUBLICATION
An adaptive optics module for deep tissue multiphoton imaging in vivo
- Authors
- Rodríguez, C., Chen, A., Rivera, J.A., Mohr, M.A., Liang, Y., Natan, R.G., Sun, W., Milkie, D.E., Bifano, T.G., Chen, X., Ji, N.
- ID
- ZDB-PUB-211116-1
- Date
- 2021
- Source
- Nature Methods 18: 1259-1264 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Bacterial Proteins
- Embryo, Nonmammalian
- Female
- Green Fluorescent Proteins
- Luminescent Proteins
- Male
- Mice
- Neuroimaging/methods*
- Optics and Photonics/methods*
- Zebrafish
- PubMed
- 34608309 Full text @ Nat. Methods
Citation
Rodríguez, C., Chen, A., Rivera, J.A., Mohr, M.A., Liang, Y., Natan, R.G., Sun, W., Milkie, D.E., Bifano, T.G., Chen, X., Ji, N. (2021) An adaptive optics module for deep tissue multiphoton imaging in vivo. Nature Methods. 18:1259-1264.
Abstract
Understanding complex biological systems requires visualizing structures and processes deep within living organisms. We developed a compact adaptive optics module and incorporated it into two- and three-photon fluorescence microscopes, to measure and correct tissue-induced aberrations. We resolved synaptic structures in deep cortical and subcortical areas of the mouse brain, and demonstrated high-resolution imaging of neuronal structures and somatosensory-evoked calcium responses in the mouse spinal cord at great depths in vivo.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping