PUBLICATION
Inter-species Transplantation of Blastocysts between Medaka and Zebrafish
- Authors
- Fuhrmann, J.F., Onistschenko, J., Centanin, L.
- ID
- ZDB-PUB-211026-13
- Date
- 2021
- Source
- Bio-protocol 11: e4166 (Journal)
- Registered Authors
- Centanin, Lazaro
- Keywords
- Blastomere transplantation, Chimeras, Medaka, Zebrafish
- MeSH Terms
- none
- PubMed
- 34692915 Full text @ Bio Protoc
Citation
Fuhrmann, J.F., Onistschenko, J., Centanin, L. (2021) Inter-species Transplantation of Blastocysts between Medaka and Zebrafish. Bio-protocol. 11:e4166.
Abstract
Transplantation of blastocysts from a donor to a host blastula constitutes a powerful experimental tool to tackle major developmental biology questions. The technique is widely implemented in diverse biological models including teleost fish, where it is typically used for intra-species blastula transplantations - i.e., labeled blastocysts into a non-labeled host to follow lineages, or mutant blastocysts into a wild-type host to address autonomous vs. non-autonomous roles of a gene of interest. We have recently implemented a protocol to transplant blastocysts between zebrafish (D. rerio) and medaka (O. latipes), two species in which blastocysts show different developmental dynamics and sizes ( Fuhrmann et al., 2020 ). We present here a detailed protocol on how to overcome the early differences in chorion structure, blastula size, and speed of development to achieve trans-species blastocyst transplantation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping