PUBLICATION
Study of the Potential Hepatoprotective Effect of Myo-Inositol and Its Influence on Zebrafish Development
- Authors
- Antonowski, T., Wiśniewski, K., Podlasz, P., Osowski, A., Wojtkiewicz, J.
- ID
- ZDB-PUB-211025-45
- Date
- 2021
- Source
- Nutrients 13(10): (Journal)
- Registered Authors
- Podlasz, Piotr
- Keywords
- acute liver damage, alcoholic liver disease, ethanol, hepatoprotection, myo-inositol, zebrafish
- MeSH Terms
-
- Animals
- Embryo, Nonmammalian/drug effects
- Embryonic Development/drug effects*
- Ethanol
- Fluorescence
- Inositol/chemistry
- Inositol/pharmacology*
- Larva/drug effects
- Larva/growth & development
- Liver/drug effects*
- Liver/pathology
- Models, Animal
- Protective Agents/pharmacology*
- Survival Analysis
- Toxicity Tests
- Zebrafish/embryology*
- PubMed
- 34684347 Full text @ Nutrients
Citation
Antonowski, T., Wiśniewski, K., Podlasz, P., Osowski, A., Wojtkiewicz, J. (2021) Study of the Potential Hepatoprotective Effect of Myo-Inositol and Its Influence on Zebrafish Development. Nutrients. 13(10):.
Abstract
Inositol is a natural substance found widely in plants. It is used in therapies for many medical cases. The aim of this study was to determine the toxicity of myo-inositol (MI) and to investigate its potential hepatoprotective character. In the first part of the study, zebrafish embryos were incubated with 5, 10, 20, 40, 60, 80, and 100 mg/mL MI. Endpoints such as survivability, hatching rate, malformation, and mobility were evaluated. Our results demonstrated that the high doses of MI lead to increased mortality and malformations and reduce the hatching rate in comparison to the control group. Moreover, low doses of this compound do not produce a negative effect on zebrafish and even have the ability to increase the hatching rate and mobility. In the second part of the study, the hepatoprotective effect of MI was tested. Zebrafish larvae from the line Tg (fabp10a:DsRed) were incubated for 24 h with 1% and 2% ethanol (EtOH), 5 mg/mL of MI with 1% EtOH, and 5 mg/mL of MI with 2% EtOH. No significant differences between the groups with EtOH and the group treated with EtOH with MI were observed. Our results suggest that MI has no positive benefits on hepatocytes of zebrafish larvae.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping