PUBLICATION
Retroposition of the Long Transcript from Multiexon IFN-β Homologs in Ancestry Vertebrate Gave Rise to the Proximal Transcription Elements of Intronless IFN-β Promoter in Humans
- Authors
- Chen, S.N., Gan, Z., Nie, P.
- ID
- ZDB-PUB-211010-1
- Date
- 2021
- Source
- Journal of immunology (Baltimore, Md. : 1950) 207(10): 2512-2520 (Journal)
- Registered Authors
- Nie, Pin
- Keywords
- none
- MeSH Terms
-
- Animals
- Chickens
- Evolution, Molecular
- Humans
- Interferon-beta/genetics*
- Introns/genetics*
- Promoter Regions, Genetic/genetics*
- Zebrafish
- PubMed
- 34625523 Full text @ J. Immunol.
Citation
Chen, S.N., Gan, Z., Nie, P. (2021) Retroposition of the Long Transcript from Multiexon IFN-β Homologs in Ancestry Vertebrate Gave Rise to the Proximal Transcription Elements of Intronless IFN-β Promoter in Humans. Journal of immunology (Baltimore, Md. : 1950). 207(10):2512-2520.
Abstract
IFN-β is a unique member of type I IFN in humans and contains four positive regulatory domains (PRDs), I-II-III-IV, in its promoter, which are docking sites for transcription factors IFN regulatory factor (IRF) 3/7, NF-κB, IRF3/7, and activating transcription factor 2/Jun proto-oncogene, respectively. In chicken IFN-β and zebrafish IFNφ1 promoters, a conserved PRD or PRD-like sequences have been reported. In this study, a type I IFN gene, named as xl-IFN1 in the amphibian model Xenopus laevis, was found to contain similar PRD-like sites, IV-III/I-II, in its promoter, and these PRD-like sites were proved to be functionally responsive to activating transcription factor 2/Jun proto-oncogene, IRF3/IRF7, and p65, respectively. The xl-IFN1, as IFNφ1 in zebrafish, was transcribed into a long and a short transcript, with the long transcript containing all of the transcriptional elements, including PRD-like sites and TATA box in its proximal promoter. A retroposition model was then proposed to explain the transcriptional conservation of IFNφ1, xl-IFN1, and IFN-β in chicken and humans.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping