PUBLICATION
Identification and functional characterization of Cofilin-1 as a new member of antimicrobial protein
- Authors
- Li, Y., Gong, Y., Chen, Y., Qu, B., Zhang, S.
- ID
- ZDB-PUB-211005-3
- Date
- 2021
- Source
- Developmental and comparative immunology 127: 104281 (Journal)
- Registered Authors
- Keywords
- Antibacterial activity, Cofilin-1, Danio rerio, Heparin-binding protein, Zebrafish
- MeSH Terms
-
- Actin Depolymerizing Factors
- Animals
- Anti-Bacterial Agents
- Staphylococcus aureus
- Zebrafish*
- Zebrafish Proteins*/genetics
- PubMed
- 34601007 Full text @ Dev. Comp. Immunol.
Citation
Li, Y., Gong, Y., Chen, Y., Qu, B., Zhang, S. (2021) Identification and functional characterization of Cofilin-1 as a new member of antimicrobial protein. Developmental and comparative immunology. 127:104281.
Abstract
Cofilin-1 (Cfl1), a member of the ADF/cofilin family, has been identified as one of differentially expressed proteins in human dendritic cells challenged with lipopolysaccharide (LPS), suggesting that it may be involved in immune response. Here we showed that zebrafish cfl1 was markedly up-regulated by LPS and LTA treatment. We also showed that zebrafish recombinant Cfl1 (rCfl1) not only bound to the Gram-negative and positive bacteria A. hydrophila and S. aureus as well as their signature molecules LPS and LTA but also inhibited the growth of the bacteria. Moreover, we found that the heparin-binding motif-containing regions of Cfl1, i.e., Cfl19-25, Cfl134-51 and Cfl1108-125, like rCfl1, were also able to bind to LPS and LTA and to inhibit the bacterial growth. rCfl1, Cfl19-25, Cfl134-51, and Cfl1108-125 were all able to cause bacterial cell destruction, to induce membrane depolarization, and to stimulate intracellular ROS production. Finally, we showed that zebrafish Cfl1 could protect developing embryos/larvae against attack by the potential pathogen A. hydrophila. These data together indicate that zebrafish Cfl1 plays an immune-relevant role as a newly-characterized antimicrobial protein.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping