PUBLICATION
Functions of SMC2 in the Development of Zebrafish Liver
- Authors
- Li, X., Song, G., Zhao, Y., Ren, J., Li, Q., Cui, Z.
- ID
- ZDB-PUB-210929-8
- Date
- 2021
- Source
- Biomedicines 9(9): (Journal)
- Registered Authors
- Cui, Zongbin, Song, Guili
- Keywords
- DNA damage, SMC2, apoptosis, liver development, zebrafish
- MeSH Terms
- none
- PubMed
- 34572426 Full text @ Biomedicines
Citation
Li, X., Song, G., Zhao, Y., Ren, J., Li, Q., Cui, Z. (2021) Functions of SMC2 in the Development of Zebrafish Liver. Biomedicines. 9(9):.
Abstract
SMC2 (structural maintenance of chromosomes 2) is the core subunit of condensins, which play a central role in chromosome organization and segregation. However, the functions of SMC2 in embryonic development remain poorly understood, due to the embryonic lethality of homozygous SMC2-/- mice. Herein, we explored the roles of SMC2 in the liver development of zebrafish. The depletion of SMC2, with the CRISPR/Cas9-dependent gene knockout approach, led to a small liver phenotype. The specification of hepatoblasts was unaffected. Mechanistically, extensive apoptosis occurred in the liver of SMC2 mutants, which was mainly associated with the activation of the p53-dependent apoptotic pathway. Moreover, an aberrant activation of a series of apoptotic pathways in SMC2 mutants was involved in the defective chromosome segregation and subsequent DNA damage. Therefore, our findings demonstrate that SMC2 is necessary for zebrafish liver development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping