PUBLICATION
Loss of tumor protein 53 protects against alcohol-induced facial malformations in mice and zebrafish
- Authors
- Fish, E.W., Tucker, S.K., Peterson, R.L., Eberhart, J.K., Parnell, S.E.
- ID
- ZDB-PUB-210929-29
- Date
- 2021
- Source
- Alcoholism, clinical and experimental research 45(10): 1965-1979 (Journal)
- Registered Authors
- Eberhart, Johann
- Keywords
- apoptosis, birth defects, craniofacial, fetal alcohol syndrome, ocular
- MeSH Terms
-
- Abnormalities, Drug-Induced/etiology*
- Abnormalities, Drug-Induced/metabolism
- Animals
- Craniofacial Abnormalities/etiology*
- Craniofacial Abnormalities/metabolism
- Ethanol/adverse effects*
- Female
- Fetal Alcohol Spectrum Disorders/metabolism*
- Male
- Mice
- Pregnancy
- Teratogenesis
- Tumor Suppressor Protein p53/metabolism*
- Zebrafish
- PubMed
- 34581462 Full text @ Alcoholism Clin. Exp. Res.
Citation
Fish, E.W., Tucker, S.K., Peterson, R.L., Eberhart, J.K., Parnell, S.E. (2021) Loss of tumor protein 53 protects against alcohol-induced facial malformations in mice and zebrafish. Alcoholism, clinical and experimental research. 45(10):1965-1979.
Abstract
Background Alcohol exposure during the gastrulation stage of development causes the craniofacial and brain malformations that define fetal alcohol syndrome. These malformations, such as a deficient philtrum, are exemplified by a loss of midline tissue and correspond, at least in part, to regionally selective cell death in the embryo. The tumor suppressor protein Tp53 is an important mechanism for cell death, but the role of Tp53 in the consequences of alcohol exposure during the gastrulation stage has yet to be examined. The current studies used mice and zebrafish to test whether genetic loss of Tp53 is a conserved mechanism to protect against the effects of early developmental stage alcohol exposure.
Methods Female mice, heterozygous for a mutation in the Tp53 gene, were mated with Tp53 heterozygous males, and the resulting embryos were exposed during gastrulation on gestational day 7 (GD 7) to alcohol (two maternal injections of 2.9 g/kg, i.p., 4 h apart) or a vehicle control. Zebrafish mutants or heterozygotes for the tp53zdf1 M214K mutation and their wild-type controls were exposed to alcohol (1.5% or 2%) beginning 6 h postfertilization (hpf), the onset of gastrulation.
Results Examination of GD 17 mice revealed that eye defects were the most common phenotype among alcohol-exposed fetuses, occurring in nearly 75% of the alcohol-exposed wild-type fetuses. Tp53 gene deletion reduced the incidence of eye defects in both the heterozygous and mutant fetuses (to about 35% and 20% of fetuses, respectively) and completely protected against alcohol-induced facial malformations. Zebrafish (4 days postfertilization) also demonstrated alcohol-induced reductions of eye size and trabeculae length that were less common and less severe in tp53 mutants, indicating a protective effect of tp53 deletion.
Conclusions These results identify an evolutionarily conserved role of Tp53 as a pathogenic mechanism for alcohol-induced teratogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping