PUBLICATION
Intrahepatic cholangiocyte regeneration from an Fgf-dependent extrahepatic progenitor niche in a zebrafish model of Alagille Syndrome
- Authors
- Zhao, C., Lancman, J.J., Yang, Y., Gates, K.P., Cao, D., Barske, L., Matalonga, J., Pan, X., He, J., Graves, A., Huisken, J., Chen, C., Dong, P.D.S.
- ID
- ZDB-PUB-210928-16
- Date
- 2021
- Source
- Hepatology (Baltimore, Md.) 75(3): 567-583 (Journal)
- Registered Authors
- Barske, Lindsey, Gates, Keith, Lancman, Joseph
- Keywords
- none
- MeSH Terms
-
- Alagille Syndrome*/genetics
- Alagille Syndrome*/metabolism
- Animals
- Bile Ducts, Extrahepatic*/growth & development
- Bile Ducts, Extrahepatic*/physiology
- Bile Ducts, Intrahepatic*/growth & development
- Bile Ducts, Intrahepatic*/physiology
- Calcium-Binding Proteins*/genetics
- Calcium-Binding Proteins*/metabolism
- Cell Transdifferentiation
- Disease Models, Animal
- Humans
- Jagged-1 Protein*/genetics
- Jagged-1 Protein*/metabolism
- Liver/growth & development
- Liver/metabolism
- Liver Regeneration/physiology*
- Receptors, Fibroblast Growth Factor/metabolism
- Receptors, Notch/metabolism*
- Signal Transduction
- Zebrafish
- Zebrafish Proteins*/genetics
- Zebrafish Proteins*/metabolism
- PubMed
- 34569629 Full text @ Hepatology
Citation
Zhao, C., Lancman, J.J., Yang, Y., Gates, K.P., Cao, D., Barske, L., Matalonga, J., Pan, X., He, J., Graves, A., Huisken, J., Chen, C., Dong, P.D.S. (2021) Intrahepatic cholangiocyte regeneration from an Fgf-dependent extrahepatic progenitor niche in a zebrafish model of Alagille Syndrome. Hepatology (Baltimore, Md.). 75(3):567-583.
Abstract
Alagille Syndrome (ALGS) is a congenital disorder caused by mutations in the Notch ligand gene, JAGGED1, leading to neonatal loss of intrahepatic duct (IHD) cells and cholestasis. Cholestasis can resolve in certain ALGS patients, suggesting regeneration of IHD cells. However, the mechanisms driving IHD cell regeneration following Jagged loss remains unclear. Here, we show that cholestasis due to developmental loss of IHD cells can be consistently phenocopied in zebrafish with compound jagged1b and jagged2b mutations or knockdown. Leveraging the transience of jagged knockdown in juvenile zebrafish, we find that resumption of Jagged expression leads to robust regeneration of IHD cells via a Notch-dependent mechanism. Combining multiple lineage tracing strategies with whole liver 3D-imaging, we demonstrate that the extrahepatic duct (EHD) is the primary source of multipotent progenitors that contribute to the regeneration, but not to the development, of IHD cells. Hepatocyte-to-IHD cell transdifferentiation is possible, but rarely detected. Progenitors in the EHD proliferate and migrate into the liver with Notch signaling loss and differentiate into IHD cells if Notch signaling increases. Tissue-specific mosaic analysis with an inducible dominant-negative Fgf receptor suggests that Fgf signaling from the surrounding mesenchymal cells maintains this extrahepatic niche by directly preventing premature differentiation and allocation of EHD progenitors to the liver. Indeed, transcriptional profiling and functional analysis of adult mouse EHD organoids uncover their distinct differentiation and proliferative potential relative to IHD organoids. CONCLUSION: Our data show that IHD cells regenerate upon resumption of Jag/Notch signaling, from multipotent progenitors originating from an Fgf-dependent extrahepatic stem cell niche. We posit that if Jagged/Notch signaling is augmented, via normal stochastic variation, gene therapy, or a Notch agonist, regeneration of IHD cells in ALGS patients may be enhanced.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping