PUBLICATION
Two-Photon Laser Ablation and In Vivo Wide-Field Imaging of Inferior Olive Neurons Revealed the Recovery of Olivocerebellar Circuits in Zebrafish
- Authors
- Hiyoshi, K., Saito, K., Fukuda, N., Matsuzaki, T., Yoshikawa, H.Y., Tsuda, S.
- ID
- ZDB-PUB-210828-30
- Date
- 2021
- Source
- International Journal of Environmental Research and Public Health 18(16): (Journal)
- Registered Authors
- Tsuda, Sachiko
- Keywords
- cerebellar reserve, climbing fiber, in vivo imaging, inferior olive, olivocerebellar circuit, two-photon laser ablation, zebrafish
- MeSH Terms
-
- Laser Therapy*
- Zebrafish
- Olivary Nucleus*
- Cerebellum/diagnostic imaging
- Animals
- Neurons
- PubMed
- 34444107 Full text @ Int. J. Environ. Res. Public Health
Citation
Hiyoshi, K., Saito, K., Fukuda, N., Matsuzaki, T., Yoshikawa, H.Y., Tsuda, S. (2021) Two-Photon Laser Ablation and In Vivo Wide-Field Imaging of Inferior Olive Neurons Revealed the Recovery of Olivocerebellar Circuits in Zebrafish. International Journal of Environmental Research and Public Health. 18(16):.
Abstract
The cerebellum, a brain region with a high degree of plasticity, is pivotal in motor control, learning, and cognition. The cerebellar reserve is the capacity of the cerebellum to respond and adapt to various disorders via resilience and reversibility. Although structural and functional recovery has been reported in mammals and has attracted attention regarding treatments for cerebellar dysfunction, such as spinocerebellar degeneration, the regulatory mechanisms of the cerebellar reserve are largely unidentified, particularly at the circuit level. Herein, we established an optical approach using zebrafish, an ideal vertebrate model in optical techniques, neuroscience, and developmental biology. By combining two-photon laser ablation of the inferior olive (IO) and long-term non-invasive imaging of "the whole brain" at a single-cell resolution, we succeeded in visualization of the morphological changes occurring in the IO neuron population and showed at a single-cell level that structural remodeling of the olivocerebellar circuit occurred in a relatively short period. This system, in combination with various functional analyses, represents a novel and powerful approach for uncovering the mechanisms of the cerebellar reserve, and highlights the potential of the zebrafish model to elucidate the organizing principles of neuronal circuits and their homeostasis in health and disease.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping