PUBLICATION

Overexpression of neuroserpin in larval and adult zebrafish shows different behavioral phenotypes

Authors
Han, S., Zhang, D., Dong, Q., Wang, X., Wang, L.
ID
ZDB-PUB-210818-2
Date
2021
Source
Neuroscience letters   762: 136175 (Journal)
Registered Authors
Wang, Xu
Keywords
anxiety, hypoactivity, neurodegeneration, neuroserpin, transgenic zebrafish
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Behavior, Animal/physiology*
  • Larva
  • Locomotion/physiology
  • Neuropeptides/metabolism*
  • Phenotype
  • Serpins/metabolism*
  • Up-Regulation
  • Zebrafish
PubMed
34400286 Full text @ Neurosci. Lett.
Abstract
Serpini1, which encodes neuroserpin, has been implicated in the development and maintenance of the nervous system. In this study, an inducible neuroserpin overexpression transgenic zebrafish was generated to investigate its role in different developmental stages. Neuroserpin overexpression was induced by doxycycline in larval and adult zebrafish respectively. Locomotion and thigmotaxis were recorded and analyzed using the ZebraBox high-throughput monitoring equipment and the ZebraLab software system. We find that Tg (serpini1) (+DOX) zebrafish larvae are more hypoactive than their wild-type counterparts at 7 day-postfertilization and anxiety-like behavior is observed in Tg (serpini1) (+DOX) adult zebrafish at 3 month-postfertilization. Furthermore, RNA-sequencing analysis reveals that neuroserpin overexpression affects neurodegeneration-related gene expression. In summary, we report that neuroserpin overexpression in larval and adult zebrafish shows different behavioral phenotypes.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes