PUBLICATION
Live imaging of adult zebrafish cardiomyocyte proliferation ex vivo
- Authors
- Honkoop, H., Nguyen, P.D., van der Velden, V.E.M., Sonnen, K.F., Bakkers, J.
- ID
- ZDB-PUB-210817-9
- Date
- 2021
- Source
- Development (Cambridge, England) 148(18): (Journal)
- Registered Authors
- Bakkers, Jeroen
- Keywords
- Cardiac, Live-imaging, Proliferation, Regeneration, Sarcomere, Zebrafish
- MeSH Terms
-
- Animals
- Animals, Genetically Modified/metabolism
- Animals, Genetically Modified/physiology
- Calpain/metabolism
- Cell Nucleus/metabolism
- Cell Nucleus/physiology
- Cell Proliferation/physiology*
- Cells, Cultured
- Cytokinesis/physiology
- Female
- Heart/physiology
- Male
- Mammals/metabolism
- Mammals/physiology
- Myocytes, Cardiac/metabolism
- Myocytes, Cardiac/physiology*
- Proteasome Endopeptidase Complex/metabolism
- Proteasome Endopeptidase Complex/physiology
- Regeneration/physiology
- Sarcomeres/metabolism
- Sarcomeres/physiology
- Zebrafish/metabolism
- Zebrafish/physiology*
- Zebrafish Proteins/metabolism
- PubMed
- 34397091 Full text @ Development
Citation
Honkoop, H., Nguyen, P.D., van der Velden, V.E.M., Sonnen, K.F., Bakkers, J. (2021) Live imaging of adult zebrafish cardiomyocyte proliferation ex vivo. Development (Cambridge, England). 148(18):.
Abstract
Zebrafish are excellent at regenerating their heart by reinitiating proliferation in pre-existing cardiomyocytes. Studying how zebrafish achieve this holds great potential in developing new strategies to boost mammalian heart regeneration. Nevertheless, the lack of appropriate live imaging tools for the adult zebrafish heart has limited detailed studies into the dynamics underlying cardiomyocyte proliferation. Here, we address this by developing a system in which cardiac slices of the injured zebrafish heart are cultured ex vivo for several days while retaining key regenerative characteristics including cardiomyocyte proliferation. In addition, we show that the cardiac slice culture system is compatible with live timelapse imaging and allows manipulation of regenerating cardiomyocytes with drugs that normally would have toxic effects that prevent its use. Finally, we use the cardiac slices to demonstrate that adult cardiomyocytes with fully assembled sarcomeres can partially disassemble their sarcomeres in a calpain and proteasome dependent manner to progress through nuclear division and cytokinesis. In conclusion, we have developed a cardiac slice culture system, which allows imaging of native cardiomyocyte dynamics in real time to discover cellular mechanisms during heart regeneration.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping