PUBLICATION
Hypoxia inducible factor 1-α is minimally involved in determining the time domains of the hypoxic ventilatory response in adult zebrafish (Danio rerio)
- Authors
- Mandic, M., Bailey, A., Perry, S.F.
- ID
- ZDB-PUB-210811-14
- Date
- 2021
- Source
- Respiratory Physiology & Neurobiology 294: 103774 (Journal)
- Registered Authors
- Perry, Steve F.
- Keywords
- Hypoxic ventilatory response (HVR), control of breathing, gene knockout, hypoxia inducible factor-1α, hypoxic ventilatory decline, zebrafish
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Hypoventilation/genetics
- Hypoventilation/physiopathology*
- Hypoxia/genetics
- Hypoxia/physiopathology*
- Hypoxia-Inducible Factor 1, alpha Subunit/physiology*
- Pulmonary Ventilation/genetics
- Pulmonary Ventilation/physiology*
- Zebrafish
- PubMed
- 34375733 Full text @ Respir. Physiol. Neurobiol.
Citation
Mandic, M., Bailey, A., Perry, S.F. (2021) Hypoxia inducible factor 1-α is minimally involved in determining the time domains of the hypoxic ventilatory response in adult zebrafish (Danio rerio). Respiratory Physiology & Neurobiology. 294:103774.
Abstract
In the current study, adult zebrafish (Danio rerio) were exposed to 72 h hypoxia (90 mmHg) to assess the time domains of the hypoxia ventilatory response (HVR) and the consequence on a subsequent more severe (40 mmHg) bout of acute hypoxia. Experiments were performed on wild-type fish and mutants in which one or both paralogs of hypoxia inducible factor-1α (hif-1α) were knocked out. Although there were subtle differences among the wild-type and knockout fish, resting fV was reestablished after 2 - 8 h of continuous hypoxia in both groups, a striking example of hypoxic ventilatory decline (HVD). When fish were subsequently exposed to more severe hypoxia, a rapid increase in fV was observed, the magnitude of which was independent of genotype or prior exposure history. During recovery, fish that had been exposed to 72 h of 90 mmHg hypoxia exhibited a pronounced undershoot in fV, which was absent in the hif-1α double knockouts. Overall, the results revealed distinct time domains of the HVR in zebrafish that were largely Hif-1α-independent.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping