PUBLICATION
Molecular characterization and mRNA expression of glutathione peroxidase and glutathione S-transferase during osmotic stress in olive flounder (Paralichthys olivaceus)
- Authors
- Choi, C.Y., An, K.W., An, M.I.
- ID
- ZDB-PUB-210806-12
- Date
- 2008
- Source
- Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 149: 330-7 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Glutathione Peroxidase/genetics*
- Glutathione Peroxidase/metabolism
- Sodium/blood
- Amino Acid Sequence
- Adaptation, Physiological/genetics
- Molecular Sequence Data
- Animals
- Cloning, Molecular
- Stress, Physiological/genetics*
- Osmotic Pressure
- DNA, Complementary/isolation & purification
- Aspartate Aminotransferases/blood
- Flounder/blood
- Flounder/genetics*
- Flounder/physiology
- Glutathione Transferase/genetics*
- Glutathione Transferase/metabolism
- Phylogeny
- RNA, Messenger/metabolism
- Chlorides/blood
- Sequence Homology, Amino Acid
- Alanine Transaminase/blood
- PubMed
- 18302988 Full text @ Comp. Biochem. Physiol. A Mol. Integr. Physiol.
Citation
Choi, C.Y., An, K.W., An, M.I. (2008) Molecular characterization and mRNA expression of glutathione peroxidase and glutathione S-transferase during osmotic stress in olive flounder (Paralichthys olivaceus). Comparative biochemistry and physiology. Part A, Molecular & integrative physiology. 149:330-7.
Abstract
Glutathione peroxidase (GPX) and glutathione S-transferase (GST) are key enzymes of cellular detoxification systems that defend cells against reactive oxygen species (ROS). In this study, we isolated the GPX and GST full-length cDNA and investigated the expression of these mRNAs from livers of olive flounder during salinity changes (35, 17.5, 8.75, 4 and 0 psu) by quantitative PCR (QPCR). GPX cDNA consists of 429 base pairs (bp) and encodes a protein of 142 amino acids. GST cDNA consists of 663 bp and encodes a protein of 220 amino acids. Both of GPX and GST mRNA expressions were the highest in 4 psu and then decreased in 0 psu. Also, the levels of Na(+) and Cl(-) decreased, and aspartate aminotransferase (AST) and alanine aminotransferase (ALT) increased during the experimental period. These findings provide molecular characterization of GPX and GST in olive flounder and suggest that GPX and GST play important roles in detoxification of ROS, thereby these maybe indicators of oxidative stress responses by salinity changes in olive flounder.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping