PUBLICATION
Construction and antitumor activity of selenium nanoparticles decorated with the polysaccharide extracted from Citrus limon (L.) Burm. f. (Rutaceae)
- Authors
- Zhou, L., Song, Z., Zhang, S., Li, Y., Xu, J., Guo, Y.
- ID
- ZDB-PUB-210801-6
- Date
- 2021
- Source
- International journal of biological macromolecules 188: 904-913 (Journal)
- Registered Authors
- Keywords
- Angiogenesis, Antitumor activity, Citrus limon polysaccharide, Selenium nanoparticles, Zebrafish model
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Antineoplastic Agents/pharmacology*
- Apoptosis/drug effects
- Citrus/chemistry*
- HeLa Cells
- Hep G2 Cells
- Humans
- Metal Nanoparticles/chemistry*
- Metal Nanoparticles/ultrastructure
- Monosaccharides/analysis
- Neovascularization, Pathologic/pathology
- Polysaccharides/chemistry
- Polysaccharides/isolation & purification*
- Selenium/pharmacology*
- Spectrophotometry, Ultraviolet
- Spectroscopy, Fourier Transform Infrared
- Xenograft Model Antitumor Assays
- Zebrafish
- PubMed
- 34331980 Full text @ Int. J. Biol. Macromol.
Citation
Zhou, L., Song, Z., Zhang, S., Li, Y., Xu, J., Guo, Y. (2021) Construction and antitumor activity of selenium nanoparticles decorated with the polysaccharide extracted from Citrus limon (L.) Burm. f. (Rutaceae). International journal of biological macromolecules. 188:904-913.
Abstract
Selenium nanoparticles (SeNPs), a potential cancer therapeutic agent, have attracted widespread attention owing to their high bioavailability and remarkable anticancer activity. Nevertheless, the poor water solubility and dispersibility of SeNPs seriously limit their applications. In the present study, we synthesized stable and individual spherical selenium nanoparticles (CL90-Tw-SeNP2) with an average diameter of approximately 79 nm using a polysaccharide extracted from Citrus limon (CL90) and Tween-80 as the decorator and stabilizers. The proportion of selenium in CL90-Tw-SeNP2 was 10.6%. CL90-Tw-SeNP2 possessed high stability and good dispersion in water for more than three months. The subsequent biological assay revealed that CL90-Tw-SeNP2 showed remarkable antitumor effects against HepG2 cells, with an IC50 value of 49.13 μg/mL, by inducing cell apoptosis. Furthermore, an in vivo zebrafish assay to explore possible applications indicated that CL90-Tw-SeNP2 could inhibit the proliferation and migration of tumors and the zebrafish angiogenesis. These results indicated that CL90-Tw-SeNP2 could be a potential agent for cancer treatment, especially against human liver hepatoma cancer.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping