PUBLICATION
The RNA helicase Ddx52 functions as a growth switch in juvenile zebrafish
- Authors
- Tseng, T.L., Wang, Y.T., Tsao, C.Y., Ke, Y.T., Lee, Y.C., Hsu, H.J., Poss, K.D., Chen, C.H.
- ID
- ZDB-PUB-210730-13
- Date
- 2021
- Source
- Development (Cambridge, England) 148(15): (Journal)
- Registered Authors
- Chen, Chen-Hui, Poss, Kenneth D.
- Keywords
- ddx52, Forward genetics, RNA helicase, Regeneration, Zebrafish
- MeSH Terms
-
- Female
- Mice
- RNA/genetics*
- Mice, Inbred C57BL
- Animals
- PubMed
- 34323273 Full text @ Development
Abstract
Vertebrate animals usually display robust growth trajectories during juvenile stages, and reversible suspension of this growth momentum by a single genetic determinant has not been reported. Here, we report a single genetic factor that is essential for juvenile growth in zebrafish. Using a forward genetic screen, we recovered a temperature-sensitive allele, pan (after Peter Pan), that suspends whole-organism growth at juvenile stages. Remarkably, even after growth is halted for a full 8-week period, pan mutants are able to resume a robust growth trajectory after release from the restrictive temperature, eventually growing into fertile adults without apparent adverse phenotypes. Positional cloning and complementation assays revealed that pan encodes a probable ATP-dependent RNA helicase (DEAD-Box Helicase 52; ddx52) that maintains the level of 47S precursor ribosomal RNA. Furthermore, genetic silencing of ddx52 and pharmacological inhibition of bulk RNA transcription similarly suspend the growth of flies, zebrafish and mice. Our findings reveal evidence that safe, reversible pauses of juvenile growth can be mediated by targeting the activity of a single gene, and that its pausing mechanism has high evolutionary conservation.
Genes / Markers
Figure Gallery (7 images)
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping