PUBLICATION
3D viscoelastic drag forces drive changes to cell shapes during organogenesis in the zebrafish embryo
- Authors
- Sanematsu, P.C., Erdemci-Tandogan, G., Patel, H., Retzlaff, E.M., Amack, J.D., Manning, M.L.
- ID
- ZDB-PUB-210718-3
- Date
- 2021
- Source
- Cells & development 168: 203718 (Journal)
- Registered Authors
- Amack, Jeffrey
- Keywords
- Cell shape, Left-right asymmetry, Organogenesis, Particle image velocimetry, Tissue mechanics, Vertex model
- MeSH Terms
-
- Animals
- Body Patterning*/physiology
- Cell Shape
- Cilia/physiology
- Organogenesis
- Zebrafish*
- PubMed
- 34273601 Full text @ Cells Dev
Citation
Sanematsu, P.C., Erdemci-Tandogan, G., Patel, H., Retzlaff, E.M., Amack, J.D., Manning, M.L. (2021) 3D viscoelastic drag forces drive changes to cell shapes during organogenesis in the zebrafish embryo. Cells & development. 168:203718.
Abstract
The left-right organizer in zebrafish embryos, Kupffer's Vesicle (KV), is a simple organ that undergoes programmed asymmetric cell shape changes that are necessary to establish the left-right axis of the embryo. We use simulations and experiments to investigate whether 3D mechanical drag forces generated by the posteriorly-directed motion of the KV through the tailbud tissue are sufficient to drive such shape changes. We develop a fully 3D vertex-like (Voronoi) model for the tissue architecture, and demonstrate that the tissue can generate drag forces and drive cell shape changes. Furthermore, we find that tailbud tissue presents a shear-thinning, viscoelastic behavior consistent with those observed in published experiments. We then perform live imaging experiments and particle image velocimetry analysis to quantify the precise tissue velocity gradients around KV as a function of developmental time. We observe robust velocity gradients around the KV, indicating that mechanical drag forces must be exerted on the KV by the tailbud tissue. We demonstrate that experimentally observed velocity fields are consistent with the viscoelastic response seen in simulations. This work also suggests that 3D viscoelastic drag forces could be a generic mechanism for cell shape change in other biological processes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping