Characterization of SR-B2a and SR-B2b genes and their ability to promote GCRV infection in grass carp (Ctenopharyngodon idellus)

Li, Y., Huang, R., Chen, L., Li, Y., Li, Y., Liao, L., He, L., Zhu, Z., Wang, Y.
Developmental and comparative immunology   124: 104202 (Journal)
Registered Authors
Zhu, Zuoyan
GCRV, Grass carp, Innate immunity, Scavenger receptor class B type 2
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Carps/genetics
  • Carps/immunology
  • Carps/virology*
  • Cell Line
  • Cell Membrane/metabolism
  • Cytoplasm/metabolism
  • Fish Diseases/genetics
  • Fish Diseases/immunology
  • Fish Diseases/virology
  • Fish Proteins/genetics
  • Fish Proteins/metabolism
  • Gene Expression
  • Immunity, Innate
  • Phylogeny
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • Reoviridae/pathogenicity*
  • Reoviridae Infections/genetics
  • Reoviridae Infections/immunology
  • Reoviridae Infections/veterinary
  • Reoviridae Infections/virology
  • Scavenger Receptors, Class B/genetics
  • Scavenger Receptors, Class B/physiology*
  • Sequence Alignment
  • Tissue Distribution
  • Viral Load/genetics
34246624 Full text @ Dev. Comp. Immunol.
Scavenger receptor class B type 2 (SR-B2) is a pattern recognition receptor involved in innate immunity in mammals; however, the immunological function of SR-Bs in fish remains unclear. In this study, the full-length cDNA sequences of SR-B2a and SR-B2b from grass carp (Ctenopharyngodon idellus) were cloned and designated as CiSR-B2a and CiSR-B2b. Multiple alignments and phylogenetic analyses deduced that CiSR-B2a and CiSR-B2b had the highest evolutionary conservation and were closely related to the zebrafish (Danio rerio) homologues, DrSR-B2a and DrSR-B2b, respectively. Both CiSR-B2a and CiSR-B2b were expressed in all the tested tissues, with the highest expression levels found in the hepatopancreas. In Ctenopharyngodon idellus kidney cells (CIK), CiSR-B2a and CiSR-B2b were mainly located in the cytoplasm, and a small amount located on the plasma membrane. After challenge with Grass Carp Reovirus (GCRV), the expression of CiSR-B2a and CiSR-B2b were significantly upregulated in the spleen (about 10.27 and 27.19 times higher than that at 0 day, p < 0.01). With CiSR-B2a or CiSR-B2b overexpressed in CIK, the relative copy number of GCRV in the cells was both significantly increased compared to that in the control group, indicating that CiSR-B2a and CiSR-B2b may be important proteins during the infection processes of GCRV.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes