PUBLICATION

Single-cell transcriptome analysis of the zebrafish embryonic trunk

Authors

Metikala, S., Casie Chetty, S., Sumanas, S.

ID

ZDB-PUB-210709-6

Date

2021

Source

PLoS One 16: e0254024 (Journal)

Registered Authors

Metikala, Sanjeev

Keywords

none

Datasets

GEO:GSE152982

MeSH Terms

Single-Cell Analysis*
Fibroblasts/cytology
Mesoderm/cytology
Mesoderm/embryology
Endothelium, Vascular/cytology
Endothelium, Vascular/embryology
Zebrafish/embryology*
Zebrafish/genetics*
Gene Expression Regulation, Developmental
Zebrafish Proteins/genetics
Zebrafish Proteins/metabolism
Cell Lineage/genetics
Torso/embryology*
Endoderm/cytology
Endoderm/embryology
Animals
Embryo, Nonmammalian/metabolism*
Gene Expression Profiling*
Erythrocytes/metabolism
Muscle, Skeletal/embryology
Muscle, Skeletal/metabolism
Ectoderm/cytology
Ectoderm/embryology

(all 23)

PubMed

34234366 Full text @ PLoS One

Abstract

During embryonic development, cells differentiate into a variety of distinct cell types and subtypes with diverse transcriptional profiles. To date, transcriptomic signatures of different cell lineages that arise during development have been only partially characterized. Here we used single-cell RNA-seq to perform transcriptomic analysis of over 20,000 cells disaggregated from the trunk region of zebrafish embryos at the 30 hpf stage. Transcriptional signatures of 27 different cell types and subtypes were identified and annotated during this analysis. This dataset will be a useful resource for many researchers in the fields of developmental and cellular biology and facilitate the understanding of molecular mechanisms that regulate cell lineage choices during development.

Genes / Markers

Figures