PUBLICATION
Single-cell transcriptome analysis of the zebrafish embryonic trunk
- Authors
- Metikala, S., Casie Chetty, S., Sumanas, S.
- ID
- ZDB-PUB-210709-6
- Date
- 2021
- Source
- PLoS One 16: e0254024 (Journal)
- Registered Authors
- Metikala, Sanjeev
- Keywords
- none
- Datasets
- GEO:GSE152982
- MeSH Terms
-
- Animals
- Cell Lineage/genetics
- Ectoderm/cytology
- Ectoderm/embryology
- Embryo, Nonmammalian/metabolism*
- Endoderm/cytology
- Endoderm/embryology
- Endothelium, Vascular/cytology
- Endothelium, Vascular/embryology
- Erythrocytes/metabolism
- Fibroblasts/cytology
- Gene Expression Profiling*
- Gene Expression Regulation, Developmental
- Mesoderm/cytology
- Mesoderm/embryology
- Muscle, Skeletal/embryology
- Muscle, Skeletal/metabolism
- Single-Cell Analysis*
- Torso/embryology*
- Zebrafish/embryology*
- Zebrafish/genetics*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 34234366 Full text @ PLoS One
Citation
Metikala, S., Casie Chetty, S., Sumanas, S. (2021) Single-cell transcriptome analysis of the zebrafish embryonic trunk. PLoS One. 16:e0254024.
Abstract
During embryonic development, cells differentiate into a variety of distinct cell types and subtypes with diverse transcriptional profiles. To date, transcriptomic signatures of different cell lineages that arise during development have been only partially characterized. Here we used single-cell RNA-seq to perform transcriptomic analysis of over 20,000 cells disaggregated from the trunk region of zebrafish embryos at the 30 hpf stage. Transcriptional signatures of 27 different cell types and subtypes were identified and annotated during this analysis. This dataset will be a useful resource for many researchers in the fields of developmental and cellular biology and facilitate the understanding of molecular mechanisms that regulate cell lineage choices during development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping