PUBLICATION
Neurulation in the posterior region of zebrafish, Danio rerio embryos
- Authors
- Gladysheva, J., Evnukova, E., Kondakova, E., Kulakova, M., Efremov, V.
- ID
- ZDB-PUB-210708-6
- Date
- 2021
- Source
- Journal of morphology 282(10): 1437-1454 (Journal)
- Registered Authors
- Keywords
- f-actin, sox2, tbxta, notochord, tail bud
- MeSH Terms
-
- Neural Tube
- Animals
- Notochord
- Zebrafish*
- Mesoderm
- Neurulation*
- PubMed
- 34233026 Full text @ J. Morphol.
Citation
Gladysheva, J., Evnukova, E., Kondakova, E., Kulakova, M., Efremov, V. (2021) Neurulation in the posterior region of zebrafish, Danio rerio embryos. Journal of morphology. 282(10):1437-1454.
Abstract
The neural tube of amniotes is formed through different mechanisms that take place in the anterior and posterior regions and involve neural plate folding or mesenchymal condensation followed by its cavitation. Meanwhile, in teleost trunk region, the neural plate forms the neural keel, while the lumen develops later. However, the data on neurulation and other morphogenetic processes in the posterior body region in Teleostei remain fragmentary. We proposed that there could be variations in the morphogenetic processes, such as cell shape changes and cell rearrangements, in the posterior region compared to the anterior one at the different stages. Here, we performed morphological and histochemical analyses of morphogenetic processes with an emphasis on neurulation in the zebrafish tail bud and posterior region. To analyze the posterior expression of sox2 and tbxta we performed WMISH. We showed that the tail bud cells of variable shapes and orientation are tightly packed, and the neural and notochord primordia develop first. The shape of the neural primordium undergoes numerous changes as a result of cell rearrangements leading to the development of the neural rod. At the prim-6 stage, the cells of the neural primordium directly form the neural rod. The neuroepithelial cells undergo sequential shape changes. At the stage of the neural rod formation, the apical regions of triangular neuroepithelial cells of the floor plate are enriched in F-actin. The neurocoel development onset is above the apical poles of neuroepithelial cells. The expression domains of sox2 and tbxta become more restricted during the development. This article is protected by copyright. All rights reserved.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping