PUBLICATION
The evaluation of zebrafish cardiovascular and behavioral functions through microfluidics
- Authors
- Subendran, S., Wang, Y.C., Lu, Y.H., Chen, C.Y.
- ID
- ZDB-PUB-210707-3
- Date
- 2021
- Source
- Scientific Reports 11: 13801 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Cardiovascular Physiological Phenomena
- Cardiovascular System/metabolism*
- Cardiovascular System/pathology
- Swimming/physiology
- Animals
- Zebrafish/physiology*
- Behavior, Animal/physiology*
- Microfluidics/instrumentation*
- Larva/physiology
- PubMed
- 34226579 Full text @ Sci. Rep.
Citation
Subendran, S., Wang, Y.C., Lu, Y.H., Chen, C.Y. (2021) The evaluation of zebrafish cardiovascular and behavioral functions through microfluidics. Scientific Reports. 11:13801.
Abstract
This study proposed a new experimental approach for the vascular and phenotype evaluation of the non-anesthetized zebrafish with representative imaging orientations for heart, pectoral fin beating, and vasculature views by means of the designed microfluidic device through inducing the optomotor response and hydrodynamic pressure control. In order to provide the visual cues for better positioning of zebrafish, computer-animated moving grids were generated by an in-house control interface which was powered by the larval optomotor response, in conjunction with the pressure suction control. The presented platform provided a comprehensive evaluation of internal circulation and the linked external behaviors of zebrafish in response to the cardiovascular parameter changes. The insights from these imaging sections was extended to identify the linkage between the cardiac parameters and behavioral endpoints. In addition, selected chemicals such as ethanol and caffeine were employed for the treatment of zebrafish. The obtained findings can be applicable for future investigation in behavioral drug screening serving as the forefront in psychopharmacological and cognition research.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping