PUBLICATION
miniSPIM-A Miniaturized Light-Sheet Microscope
- Authors
- Hedde, P.N.
- ID
- ZDB-PUB-210702-11
- Date
- 2021
- Source
- ACS sensors 6(7): 2654-2663 (Journal)
- Registered Authors
- Keywords
- bacteria detection, fluorescence fluctuation spectroscopy, image correlation spectroscopy, light-sheet microscopy, optical sectioning, particle detection, selective plane illumination microscopy, three-dimensional imaging
- MeSH Terms
-
- Animals
- Imaging, Three-Dimensional*
- Microscopy, Fluorescence
- Smartphone
- Zebrafish*
- PubMed
- 34197085 Full text @ ACS Sens
Citation
Hedde, P.N. (2021) miniSPIM-A Miniaturized Light-Sheet Microscope. ACS sensors. 6(7):2654-2663.
Abstract
The miniSPIM is a miniaturized light-sheet microscope that enables imaging with optical sectioning on mobile camera devices such as smartphones and single-board computers. Applications of the miniSPIM include biosensing, field research, and education where maximum portability and robustness, low power consumption, and low cost are key. Here, it is shown how all of the components of a simple light-sheet microscope can be integrated within a footprint smaller than the average smartphone. Example applications include the quantification of the motion of microparticles and bacteria in fluids, the characterization of solvent polarity based on spectral shifts of the lipid probe Nile Red, and three-dimensional (3D) and time-lapse autofluorescence imaging of a live zebrafish embryo.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping