PUBLICATION
Edwardsiella ictaluri in a Colony of Zebrafish (Danio rerio) used in a Teaching Laboratory
- Authors
- Sun, F.J., Crim, M.J., Leblanc, M.
- ID
- ZDB-PUB-210701-2
- Date
- 2021
- Source
- Comparative medicine 71(4): 318-322 (Journal)
- Registered Authors
- Crim, Marcus J.
- Keywords
- none
- MeSH Terms
-
- Animals
- Edwardsiella ictaluri
- Enterobacteriaceae Infections*/veterinary
- Fish Diseases*
- Laboratories
- Zebrafish
- PubMed
- 34187617 Full text @ Comp. Med.
Citation
Sun, F.J., Crim, M.J., Leblanc, M. (2021) Edwardsiella ictaluri in a Colony of Zebrafish (Danio rerio) used in a Teaching Laboratory. Comparative medicine. 71(4):318-322.
Abstract
A small colony of zebrafish (Danio rerio) experienced 30% acute mortality within a few days after receipt from a commercial source. A few fish presented with small areas of raised scales or tissue necrosis, primarily near the caudal peduncle. Edwardsiella ictaluri (E. ictaluri) was identified by real-time PCR of pooled zebrafish and swabs of the pre-filter and fine filter pads, with subsequent sequence analysis. E. ictaluri is most commonly associated with an enteric septicemia in catfish species and can have significant economic impact on commercial catfish fisheries. However, several references report naturally occurring E. ictaluri infection of nonictalurid fishes, including zebrafish. Ours is the first report demonstrating the use of environmental sampling to identify E. ictaluri in a zebrafish colony by real-time PCR. Moreover, our report indicates that E.ictaluri is a relevant disease for institutions using zebrafish as research species and emphasizes the importance of carefully considering importation and quarantine practices.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping