PUBLICATION
Zebrafish otud6b Negatively Regulates Antiviral Responses by Suppressing K63-Linked Ubiquitination of irf3 and irf7
- Authors
- Zhou, Z., Cai, X., Zhu, J., Li, Z., Yu, G., Liu, X., Ouyang, G., Xiao, W.
- ID
- ZDB-PUB-210630-4
- Date
- 2021
- Source
- Journal of immunology (Baltimore, Md. : 1950) 207(1): 244-256 (Journal)
- Registered Authors
- Li, Zhi, Ouyang, Gang, Xiao, Wuhan, Yu, Guangqing
- Keywords
- none
- MeSH Terms
-
- Animals
- Carps/immunology*
- Carps/virology
- Cell Line
- Interferon Regulatory Factor-3/immunology*
- Interferon Regulatory Factors/immunology*
- Lysine/immunology*
- Ubiquitination
- Viremia/immunology*
- Viremia/virology
- Zebrafish
- Zebrafish Proteins/genetics
- Zebrafish Proteins/immunology*
- PubMed
- 34183367 Full text @ J. Immunol.
Citation
Zhou, Z., Cai, X., Zhu, J., Li, Z., Yu, G., Liu, X., Ouyang, G., Xiao, W. (2021) Zebrafish otud6b Negatively Regulates Antiviral Responses by Suppressing K63-Linked Ubiquitination of irf3 and irf7. Journal of immunology (Baltimore, Md. : 1950). 207(1):244-256.
Abstract
Ovarian tumor domain-containing 6B (OTUD6B) belongs to the OTU deubiquitylating enzyme family. In this study, we report that zebrafish otud6b is induced upon viral infection, and overexpression of otud6b suppresses cellular antiviral response. Disruption of otud6b in zebrafish increases the survival rate upon spring viremia of carp virus and grass carp reovirus exposure. Further assays indicate that otud6b interacts with irf3 and irf7 and diminishes traf6-mediated K63-linked polyubiquitination of irf3 and irf7. In addition, the OTU domain is required for otud6b to repress IFN-1 activation and K63-linked polyubiquitination of irf3 and irf7. Moreover, otud6b also attenuates tbk1 to bind to irf3 and irf7, resulting in the impairment of irf3 and irf7 phosphorylation. This study provides, to our knowledge, novel insights into otud6b function and sheds new lights on the regulation of irf3 and irf7 by deubiquitination in IFN-1 signaling.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping