PUBLICATION
Schaftoside Suppresses Pentylenetetrazol-Induced Seizures in Zebrafish via Suppressing Apoptosis, Modulating Inflammation, and Oxidative Stress
- Authors
- Dang, J., Paudel, Y.N., Yang, X., Ren, Q., Zhang, S., Ji, X., Liu, K., Jin, M.
- ID
- ZDB-PUB-210616-29
- Date
- 2021
- Source
- ACS Chemical Neuroscience 12(13): 2542-2552 (Journal)
- Registered Authors
- Keywords
- Anti-inflammation, Antiapoptosis, Antioxidative stress, Epilepsy, Traditional Chinese medicines
- MeSH Terms
-
- Animals
- Apoptosis
- Glycosides
- Inflammation/drug therapy
- Oxidative Stress
- Pentylenetetrazole*/toxicity
- Seizures/chemically induced
- Seizures/drug therapy
- Zebrafish*
- PubMed
- 34128378 Full text @ ACS Chem. Neurosci.
Citation
Dang, J., Paudel, Y.N., Yang, X., Ren, Q., Zhang, S., Ji, X., Liu, K., Jin, M. (2021) Schaftoside Suppresses Pentylenetetrazol-Induced Seizures in Zebrafish via Suppressing Apoptosis, Modulating Inflammation, and Oxidative Stress. ACS Chemical Neuroscience. 12(13):2542-2552.
Abstract
The lack of disease-modifying therapeutic strategies against epileptic seizures has caused a surge in preclinical research focused on exploring and developing novel therapeutic candidates for epilepsy. Compounds from traditional Chinese medicines (TCMs) have gained much attention for a plethora of neurological diseases, including epilepsy. Herein, for the first time, we evaluated the anticonvulsive effects of schaftoside (SS), a TCM, on pentylenetetrazol (PTZ)-induced epileptic seizures in zebrafish and examined the underlying mechanisms. We observed that SS pretreatments significantly suppressed seizure-like behavior and prolonged the onset of seizures. Zebrafish larvae pretreated with SS demonstrated downregulation of c-fos expression during seizures. PTZ-induced upregulation of apoptotic cells was decreased upon pretreatment with SS. Inflammatory phenomena during seizure progression including the upregulation of interleukin 6 (IL-6), interleukin 1 beta (IL-1β), and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) were downregulated upon pretreatment with SS. The PTZ-induced recruitment of immunocytes was in turn reduced upon SS pretreatment. Moreover, SS pretreatment modulated oxidative stress, as demonstrated by decreased levels of catalase (CAT) and increased levels of glutathione peroxidase-1a (GPx1a) and manganese superoxide dismutase (Mn-SOD). However, pretreatment with SS modulated the PTZ-induced downregulation of the relative enzyme activity of CAT, GPx, and SOD. Hence, our findings suggest that SS pretreatment ameliorates PTZ-induced seizures, suppresses apoptosis, and downregulates the inflammatory response and oxidative stress, which potentially protect against further seizures in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping