PUBLICATION
Gene Expression of CRAL_TRIO Family Proteins modulated by Vitamin E Deficiency in Zebrafish (Danio Rerio)
- Authors
- Watt, A.T., Head, B., Leonard, S.W., Tanguay, R.L., Traber, M.G.
- ID
- ZDB-PUB-210616-2
- Date
- 2021
- Source
- The Journal of Nutritional Biochemistry 97: 108801 (Journal)
- Registered Authors
- Tanguay, Robyn L.
- Keywords
- Alpha-tocopherol transfer protein (α-TTP), CRAL_TRIO, SEC14, choline, ptpn9a
- MeSH Terms
-
- Carrier Proteins/genetics*
- Carrier Proteins/metabolism
- Antioxidants
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- Malondialdehyde/metabolism
- alpha-Tocopherol/metabolism
- Lipid Metabolism/genetics
- Vitamin E Deficiency/genetics*
- Vitamin E Deficiency/metabolism
- Zebrafish
- Gene Expression*
- Animals
- Liver/metabolism*
- Choline Kinase/genetics
- Choline Kinase/metabolism
- Protein Tyrosine Phosphatases, Non-Receptor/genetics*
- Protein Tyrosine Phosphatases, Non-Receptor/metabolism
- PubMed
- 34119630 Full text @ J. Nutr. Biochem.
Citation
Watt, A.T., Head, B., Leonard, S.W., Tanguay, R.L., Traber, M.G. (2021) Gene Expression of CRAL_TRIO Family Proteins modulated by Vitamin E Deficiency in Zebrafish (Danio Rerio). The Journal of Nutritional Biochemistry. 97:108801.
Abstract
An evaluation of the impact of vitamin E deficiency on expression of the alpha-tocopherol transfer protein (α-TTP) and related CRAL_TRIO genes was undertaken using livers from adult zebrafish based on the hypothesis that increased lipid peroxidation would modulate gene expression. Zebrafish were fed either a vitamin E sufficient (E+) or deficient (E-) diet for 9 months, then fish were euthanized, and livers were harvested. Livers from the E+ relative to E- fish contained 40-times more α-tocopherol (P<0.0001) and one fourth the malondialdehyde (P = 0.0153). RNA was extracted from E+ and E- livers, then subject to evaluation of gene expression of ttpa and other genes of the CRAL_TRIO family, genes of antioxidant markers, and genes related to lipid metabolism. Ttpa expression was not altered by vitamin E status. However, one member of the CRAL_TRIO family, tyrosine-protein phosphatase non-receptor type 9 gene (ptpn9a), showed a 2.4-fold increase (P=0.029) in E- relative to E+ livers. Further, we identified that the gene for choline kinase alpha (chka) showed a 3.0-fold increase (P=0.010) in E- livers. These outcomes are consistent with our previous findings that show vitamin E deficiency increased lipid peroxidation causing increases in phospholipid turnover.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping