PUBLICATION
Using zebrafish as a model to assess the individual and combined effects of sub-lethal waterborne and dietary zinc exposure during development
- Authors
- Puar, P., Naderi, M., Niyogi, S., Kwong, R.W.M.
- ID
- ZDB-PUB-210602-13
- Date
- 2021
- Source
- Environmental pollution (Barking, Essex : 1987) 284: 117377 (Journal)
- Registered Authors
- Keywords
- Developing fish, Metal regulation, ZIP, Zebrafish, Zinc
- MeSH Terms
-
- Animals
- Cation Transport Proteins*
- Endoplasmic Reticulum/metabolism
- Larva/metabolism
- Water Pollutants, Chemical*/toxicity
- Zebrafish/metabolism
- Zinc/metabolism
- Zinc/toxicity
- PubMed
- 34062438 Full text @ Environ. Pollut.
Citation
Puar, P., Naderi, M., Niyogi, S., Kwong, R.W.M. (2021) Using zebrafish as a model to assess the individual and combined effects of sub-lethal waterborne and dietary zinc exposure during development. Environmental pollution (Barking, Essex : 1987). 284:117377.
Abstract
The present research used zebrafish (5-28 days post-fertilization; dpf) as a model organism to investigate the effects of chronic exposure to environmentally relevant sub-lethal concentrations of waterborne (261 μg/L) and dietary zinc (Zn) (1500 mg Zn/kg dw), either independently or simultaneously, during development. The results showed that whole body contents of Zn were increased in all Zn treatment groups, with the highest accumulation of Zn observed in larvae simultaneously exposed to elevated waterborne and dietary Zn. In addition, exposure to elevated levels of Zn, either through the water or the diet, led to a decrease in whole body calcium (Ca) contents at 28 dpf. The findings also suggested that exposure to elevated levels of Zn resulted in a significant reduction in whole body manganese (Mn) contents. More importantly, the magnitude of decrease in Mn contents by Zn exposure was markedly higher than that in Ca and appeared to mirror the increases in whole body Zn accumulation. These results indicate that Mn regulation is more sensitive than Ca to disruption by Zn exposure in developing fish. Further examination of the Zrt-Irt-Like Protein (ZIP) family of transporters using droplet digital PCR technologies revealed that several zip transporters exhibited temporal and exposure route-specific changes following Zn exposure. In particular, the level of zip4 was influenced by Zn exposure regardless of the exposure routes, while changes in zip7 and zip8 levels were predominantly driven by waterborne exposure. Overall, our findings demonstrated that zebrafish during the developmental periods are sensitive to elevated levels of Zn seen in the environment, particularly following co-exposures to waterborne and dietary Zn. Future toxicological assessment of elevated Zn exposure should consider both the exposure routes and the life stages of fish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping