PUBLICATION
Zebrafish twist2/dermo1 regulates scale shape and scale organization during skin development and regeneration
- Authors
- Jacob, T., Chakravarty, A., Panchal, A., Patil, M., Ghodadra, G., Sudhakaran, J., Nuesslein-Volhard, C.
- ID
- ZDB-PUB-210519-8
- Date
- 2021
- Source
- Cells & development 166: 203684 (Journal)
- Registered Authors
- Jacob, Tressa Panikulangara
- Keywords
- Metamorphosis, Regeneration, Scales, Wnt signaling, twist2/dermo1
- MeSH Terms
-
- Animal Scales/anatomy & histology*
- Animals
- Base Sequence
- Gene Expression Regulation, Developmental
- Genotype
- Mutation/genetics
- Phenotype
- Regeneration/physiology*
- Skin/embryology*
- Twist-Related Protein 2/genetics
- Twist-Related Protein 2/metabolism*
- Wnt Signaling Pathway
- Zebrafish/embryology*
- Zebrafish/genetics*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 33994357 Full text @ Cells Dev
Citation
Jacob, T., Chakravarty, A., Panchal, A., Patil, M., Ghodadra, G., Sudhakaran, J., Nuesslein-Volhard, C. (2021) Zebrafish twist2/dermo1 regulates scale shape and scale organization during skin development and regeneration. Cells & development. 166:203684.
Abstract
Scales are skin appendages in fishes that evolutionarily predate feathers in birds and hair in mammals. Zebrafish scales are dermal in origin and develop during metamorphosis. Understanding regulation of scale development in zebrafish offers an exciting possibility of unraveling how the mechanisms of skin appendage formation evolved in lower vertebrates and whether these mechanisms remained conserved in birds and mammals. Here we have investigated the expression and function of twist 2/dermo1 gene - known for its function in feather and hair formation - in scale development and regeneration. We show that of the four zebrafish twist paralogues, twist2/dermo1 and twist3 are expressed in the scale forming cells during scale development. Their expression is also upregulated during scale regeneration. Our knockout analysis reveals that twist2/dermo1 gene functions in the maintenance of the scale shape and organization during development as well as regeneration. We further show that the expression of twist2/dermo1 and twist3 is regulated by Wnt signaling. Our results demonstrate that the function of twist2/dermo1 in skin appendage formation, presumably under regulation of Wnt signaling, originated during evolution of basal vertebrates.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping