PUBLICATION

Potential role for the tumor suppressor CYLD in brain and notochord development

Authors
Li, T., Wang, Y., Li, D., Zhou, J., Zhang, B., He, X.
ID
ZDB-PUB-210514-7
Date
2021
Source
Thoracic cancer   12(12): 1900-1908 (Journal)
Registered Authors
Zhang, Bo
Keywords
CYLD, TALEN, brain, cylindromatosis, tumor suppressor
MeSH Terms
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • Animals
  • Humans
  • Brain/growth & development*
  • Brain/metabolism
  • Deubiquitinating Enzyme CYLD/genetics
  • Deubiquitinating Enzyme CYLD/metabolism*
  • Notochord/growth & development*
  • Notochord/metabolism
  • Zebrafish
(all 11)
PubMed
33982884 Full text @ Thorac Cancer
Abstract
Background The cylindromatosis (CYLD) tumor suppressor is a microtubule-associated deubiquitinase that plays a critical role in the regulation of cell signaling and contributes to a variety of physiological and pathological processes. However, the functions of CYLD in zebrafish are less well known, particularly with regard to their development and physiology. In this context, we investigated the loss of function of CYLD in zebrafish via transcription activator-like effector nuclease (TALEN)-based gene deletion.
Methods Semi-quantitative RT-PCR was used to quantify CYLD mRNA expression in zebrafish embryos at various developmental stages. We also performed whole-mount in situ hybridization to further assess the dynamic expression and distribution of CYLD in the entire zebrafish embryos at different stages. In addition, we deleted CYLD in zebrafish with TALENs to investigate its potential impact on embryonic development.
Results The expression of CYLD mRNA varied during early embryonic development. The CYLD mRNA localized to the brain and notochord of developing zebrafish embryos. Homozygous deletion of CYLD resulted in embryonic death before 8 h post-fertilization.
Conclusions CYLD appears to play an important role in central nervous system development in zebrafish. Although severe embryonic death restricted analysis of homozygous mutants, further research into the role of CYLD in central nervous system development is warranted.
Genes / Markers
Figures
Figure Gallery (6 images)
Show all Figures
Expression
Phenotype
Fish Conditions Stage Phenotype Figure
cyldzf3543/+standard conditionsAdult
cyldzf3543/+standard conditionsAdult
cyldzf3543/zf3543standard conditions75%-epiboly
1 - 3 of 3
Show
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
zf3543
    		Small Deletion
    1 - 1 of 1
    Show
    Human Disease / Model
    No data available
    Sequence Targeting Reagents
    Target Reagent Reagent Type
    cyldTALEN1-cyldTALEN
    cyldTALEN2-cyldTALEN
    1 - 2 of 2
    Show
    Fish
    Fish
    cyldzf3543/+
    cyldzf3543/zf3543
    WT
    1 - 3 of 3
    Show
    Antibodies
    No data available
    Orthology
    No data available
    Engineered Foreign Genes
    No data available
    Mapping
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    Citation
    Li, T., Wang, Y., Li, D., Zhou, J., Zhang, B., He, X. (2021) Potential role for the tumor suppressor CYLD in brain and notochord development. Thoracic cancer. 12(12):1900-1908.