ZFIN ID: ZDB-PUB-210512-1
Photoactivatable metabolic warheads enable precise and safe ablation of target cells in vivo
Benson, S., de Moliner, F., Fernandez, A., Kuru, E., Asiimwe, N.L., Lee, J.S., Hamilton, L., Sieger, D., Bravo, I.R., Elliot, A.M., Feng, Y., Vendrell, M.
Date: 2021
Source: Nature communications   12: 2369 (Journal)
Registered Authors: Feng, Yi, Sieger, Dirk
Keywords: none
MeSH Terms:
  • Animals
  • Bacterial Infections/drug therapy*
  • Coculture Techniques
  • Fluorescent Dyes/administration & dosage*
  • Fluorescent Dyes/adverse effects
  • Fluorescent Dyes/chemistry
  • Fluorescent Dyes/radiation effects
  • Glioblastoma/drug therapy*
  • Glioblastoma/pathology
  • Humans
  • Intravital Microscopy
  • Light
  • Microbial Sensitivity Tests
  • Microscopy, Confocal
  • Microscopy, Fluorescence
  • Organoselenium Compounds/administration & dosage*
  • Organoselenium Compounds/adverse effects
  • Organoselenium Compounds/chemistry
  • Organoselenium Compounds/radiation effects
  • Photochemotherapy/methods*
  • Spheroids, Cellular
  • Xenograft Model Antitumor Assays
  • Zebrafish
PubMed: 33888691 Full text @ Nat. Commun.
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ABSTRACT
Photoactivatable molecules enable ablation of malignant cells under the control of light, yet current agents can be ineffective at early stages of disease when target cells are similar to healthy surrounding tissues. In this work, we describe a chemical platform based on amino-substituted benzoselenadiazoles to build photoactivatable probes that mimic native metabolites as indicators of disease onset and progression. Through a series of synthetic derivatives, we have identified the key chemical groups in the benzoselenadiazole scaffold responsible for its photodynamic activity, and subsequently designed photosensitive metabolic warheads to target cells associated with various diseases, including bacterial infections and cancer. We demonstrate that versatile benzoselenadiazole metabolites can selectively kill pathogenic cells - but not healthy cells - with high precision after exposure to non-toxic visible light, reducing any potential side effects in vivo. This chemical platform provides powerful tools to exploit cellular metabolic signatures for safer therapeutic and surgical approaches.
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