PUBLICATION
Structural analysis and biological effects of a neutral polysaccharide from the fruits of Rosa laevigata
- Authors
- Zhang, J., Song, Z., Li, Y., Zhang, S., Bao, J., Wang, H., Dong, C., Ohizumi, Y., Xu, J., Guo, Y.
- ID
- ZDB-PUB-210511-6
- Date
- 2021
- Source
- Carbohydrate Polymers 265: 118080 (Journal)
- Registered Authors
- Keywords
- 2′, 7′-Dichlorofluorescein diacetate (PubChem CID: 104913), 3-Amino, 4-aminomethyl-2′, 7′-difluorescein diacetate (PubChem CID: 2762646), Antitumor activity, Chloroform (PubChem CID: 6212), Dimethyl sulfoxide (PubChem CID: 679), Immunoregulatory activity, Methyl iodide (PubChem CID: 6328), Neutral polysaccharide, Rosa laevigata, Sodium borohydride (PubChem CID: 4311764), Sodium chloride (PubChem CID: 5234), Thiazolyl blue tetrazolium bromide (PubChem CID: 64965), Tricaine (PubChem CID: 261501), Trifluoroacetic acid (PubChem CID: 6422), Zebrafish xenograft model
- MeSH Terms
-
- Animals
- Antineoplastic Agents/pharmacology
- Cell Movement/drug effects
- Cell Proliferation/drug effects
- Fruit/chemistry*
- Humans
- Immunologic Factors/pharmacology
- Immunomodulation/drug effects
- K562 Cells
- Mice
- Molecular Weight
- Monosaccharides/analysis
- Nitric Oxide/analysis
- Polysaccharides/chemistry*
- Polysaccharides/pharmacology*
- RAW 264.7 Cells
- Reactive Oxygen Species
- Rosa/chemistry*
- Zebrafish
- PubMed
- 33966844 Full text @ Carbohydr. Polym.
Citation
Zhang, J., Song, Z., Li, Y., Zhang, S., Bao, J., Wang, H., Dong, C., Ohizumi, Y., Xu, J., Guo, Y. (2021) Structural analysis and biological effects of a neutral polysaccharide from the fruits of Rosa laevigata. Carbohydrate Polymers. 265:118080.
Abstract
A neutral water-soluble polysaccharide (RLP50-2) was extracted and purified from the fruits of Rosa laevigata. The absolute molecular weight was determined as 1.26 × 104 g/mol. Monosaccharide composition analysis showed that RLP50-2 mainly consisted of glucose, arabinose, and galactose. Structural analysis revealed that RLP50-2 consisted of →5)-α-L-Araf-(1→, →2,5)-α-L-Araf-(1→, →3,5)-α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →6)-α-D-Glcp-(1→, →3,6)-β-D-Glcp-(1→, →4)-α-D-Galp-(1→, →6)-β-D-Galp-(1→, →2)-β-D-Xylp-(1→, terminal α-L-arabinose, and terminal β-D-mannose. Biological assays showed that RLP50-2 had immunomodulatory activities using cell and zebrafish models. Moreover, RLP50-2 showed significantly antitumor activities by inhibiting tumor cell proliferation and migration and blocking angiogenesis. These results suggested that RLP50-2 could be developed as a potential immunomodulatory agent or antitumor candidate drug in biomedicine field.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping