PUBLICATION
Preventive Effects of Green Tea Extract against Obesity Development in Zebrafish
- Authors
- Zang, L., Shimada, Y., Nakayama, H., Katsuzaki, H., Kim, Y., Chu, D.C., Juneja, L.R., Kuroyanagi, J., Nishimura, N.
- ID
- ZDB-PUB-210506-1
- Date
- 2021
- Source
- Molecules 26(9): (Journal)
- Registered Authors
- Keywords
- Mibyo, RNA-seq, green tea extract, obesity, visceral adipose tissue, zebrafish
- MeSH Terms
-
- Adipose Tissue/drug effects
- Adipose Tissue/metabolism
- Animals
- Biomarkers
- Intra-Abdominal Fat/drug effects
- Intra-Abdominal Fat/metabolism
- Lipid Metabolism/drug effects
- Obesity/drug therapy
- Obesity/metabolism
- Organ Size/drug effects
- Plant Extracts/chemistry
- Plant Extracts/pharmacology*
- Signal Transduction/drug effects
- Tea/chemistry*
- Zebrafish
- PubMed
- 33946279 Full text @ Molecules
Citation
Zang, L., Shimada, Y., Nakayama, H., Katsuzaki, H., Kim, Y., Chu, D.C., Juneja, L.R., Kuroyanagi, J., Nishimura, N. (2021) Preventive Effects of Green Tea Extract against Obesity Development in Zebrafish. Molecules. 26(9):.
Abstract
Various natural products (NPs) have been used to treat obesity and related diseases. However, the best way to fight obesity is preventive, with accurate body weight management through exercise, diet, or bioactive NPs to avoid obesity development. We demonstrated that green tea extract (GTE) is an anti-obesity NP using a zebrafish obesity model. Based on a hypothesis that GTE can prevent obesity, the objective of this study was to assess GTE's ability to attenuate obesity development. Juvenile zebrafish were pretreated with GTE for seven days before obesity induction via a high-fat diet; adult zebrafish were pretreated with GTE for two weeks before obesity induction by overfeeding. As a preventive intervention, GTE significantly decreased visceral adipose tissue accumulation in juveniles and ameliorated visceral adiposity and plasma triglyceride levels in adult zebrafish obesity models. RNA sequencing analysis was performed using liver tissues from adult obese zebrafish, with or without GTE administration, to investigate the underlying molecular mechanism. Transcriptome analysis revealed that preventive GTE treatment affects several pathways associated with anti-obesity regulation, including activation of STAT and downregulation of CEBP signaling pathways. In conclusion, GTE could be used as a preventive agent against obesity.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping