PUBLICATION
CRISPR prime editing with ribonucleoprotein complexes in zebrafish and primary human cells
- Authors
- Petri, K., Zhang, W., Ma, J., Schmidts, A., Lee, H., Horng, J.E., Kim, D.Y., Kurt, I.C., Clement, K., Hsu, J.Y., Pinello, L., Maus, M.V., Joung, J.K., Yeh, J.J.
- ID
- ZDB-PUB-210501-47
- Date
- 2021
- Source
- Nature Biotechnology 40(2): 189-193 (Other)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- CRISPR-Cas Systems/genetics
- Clustered Regularly Interspaced Short Palindromic Repeats*/genetics
- Gene Editing
- HEK293 Cells
- Humans
- RNA, Guide, Kinetoplastida/genetics
- Ribonucleoproteins/genetics
- Zebrafish*/genetics
- PubMed
- 33927418 Full text @ Nat Biotechnol.
Citation
Petri, K., Zhang, W., Ma, J., Schmidts, A., Lee, H., Horng, J.E., Kim, D.Y., Kurt, I.C., Clement, K., Hsu, J.Y., Pinello, L., Maus, M.V., Joung, J.K., Yeh, J.J. (2021) CRISPR prime editing with ribonucleoprotein complexes in zebrafish and primary human cells. Nature Biotechnology. 40(2):189-193.
Abstract
Prime editors have been delivered using DNA or RNA vectors. Here we demonstrate prime editing with purified ribonucleoprotein complexes. We introduced somatic mutations in zebrafish embryos with frequencies as high as 30% and demonstrate germline transmission. We also observed unintended insertions, deletions and prime editing guide RNA (pegRNA) scaffold incorporations. In HEK293T and primary human T cells, prime editing with purified ribonucleoprotein complexes introduced desired edits with frequencies of up to 21 and 7.5%, respectively.
Errata / Notes
Corrected by: ZDB-PUB-210515-6
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping