PUBLICATION

Generation of a Novel Transgenic Zebrafish for Studying Adipocyte Development and Metabolic Control

Authors
Mao, Y., Hong, K.H., Liao, W., Li, L., Kim, S.J., Xiong, Y., Nam, I.K., Choe, S.K., Kwak, S.A.
ID
ZDB-PUB-210501-32
Date
2021
Source
International Journal of Molecular Sciences   22(8): (Journal)
Registered Authors
Choe, Seong-Kyu
Keywords
adipose tissue, live imaging, obesity, transgenic zebrafish
MeSH Terms
  • Green Fluorescent Proteins/metabolism
  • Animals, Genetically Modified
  • Transgenes
  • Promoter Regions, Genetic/genetics
  • Adipose Tissue/metabolism
  • Animal Nutritional Physiological Phenomena
  • Adipocytes/cytology*
  • Adipocytes/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • Larva/genetics
  • Larva/metabolism
  • Zebrafish/genetics*
  • Zebrafish/metabolism*
  • Cell Shape
  • Animals
(all 16)
PubMed
33924375 Full text @ Int. J. Mol. Sci.
Abstract
Zebrafish have become a popular animal model for studying various biological processes and human diseases. The metabolic pathways and players conserved among zebrafish and mammals facilitate the use of zebrafish to understand the pathological mechanisms underlying various metabolic disorders in humans. Adipocytes play an important role in metabolic homeostasis, and zebrafish adipocytes have been characterized. However, a versatile and reliable zebrafish model for long-term monitoring of adipose tissues has not been reported. In this study, we generated stable transgenic zebrafish expressing enhanced green fluorescent protein (EGFP) in adipocytes. The transgenic zebrafish harbored adipose tissues that could be detected using GFP fluorescence and the morphology of single adipocyte could be investigated in vivo. In addition, we demonstrated the applicability of this model to the long-term in vivo imaging of adipose tissue development and regulation based on nutrition. The transgenic zebrafish established in this study may serve as an excellent tool to advance the characterization of white adipose tissue in zebrafish, thereby aiding the development of therapeutic interventions to treat metabolic diseases in humans.
Genes / Markers
Figures
Figure Gallery (4 images)
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
wk3TgTransgenic Insertion
    1 - 1 of 1
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    Human Disease / Model
    No data available
    Sequence Targeting Reagents
    No data available
    Fish
    No data available
    Antibodies
    No data available
    Orthology
    No data available
    Engineered Foreign Genes
    Marker Marker Type Name
    EGFPEFGEGFP
    1 - 1 of 1
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    Mapping
    No data available
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    Citation
    Mao, Y., Hong, K.H., Liao, W., Li, L., Kim, S.J., Xiong, Y., Nam, I.K., Choe, S.K., Kwak, S.A. (2021) Generation of a Novel Transgenic Zebrafish for Studying Adipocyte Development and Metabolic Control. International Journal of Molecular Sciences. 22(8):.