PUBLICATION
A Novel SLC5A5 Variant Reveals the Crucial Role of Kinesin Light Chain 2 in Thyroid Hormonogenesis
- Authors
- Martín, M., Modenutti, C.P., Gil Rosas, M.L., Peyret, V., Geysels, R.C., Bernal Barquero, C.E., Sobrero, G., Muñoz, L., Signorino, M., Testa, G., Miras, M.B., Masini-Repiso, A.M., Calcaterra, N.B., Coux, G., Carrasco, N., Martí, M.A., Nicola, J.P.
- ID
- ZDB-PUB-210430-6
- Date
- 2021
- Source
- The Journal of clinical endocrinology and metabolism 106(7): 1867-1881 (Journal)
- Registered Authors
- Calcaterra, Nora
- Keywords
- Dyshormonogenic Congenital Hypothyroidism, Impaired Transport to the Plasma Membrane, Iodide Transport Defect, Kinesin Light Chain 2, Sodium/Iodide Symporter, Tryptophan-acidic motif
- MeSH Terms
-
- Animals
- Congenital Hypothyroidism/genetics*
- Humans
- Infant, Newborn
- Iodides/metabolism
- Male
- Metabolism, Inborn Errors/genetics*
- Microtubule-Associated Proteins/metabolism*
- Mutation, Missense
- Phenotype
- Rats
- Symporters/genetics*
- Thyroid Gland/metabolism
- Thyroid Hormones/metabolism*
- PubMed
- 33912899 Full text @ J. Clin. Endocrinol. Metab.
Citation
Martín, M., Modenutti, C.P., Gil Rosas, M.L., Peyret, V., Geysels, R.C., Bernal Barquero, C.E., Sobrero, G., Muñoz, L., Signorino, M., Testa, G., Miras, M.B., Masini-Repiso, A.M., Calcaterra, N.B., Coux, G., Carrasco, N., Martí, M.A., Nicola, J.P. (2021) A Novel SLC5A5 Variant Reveals the Crucial Role of Kinesin Light Chain 2 in Thyroid Hormonogenesis. The Journal of clinical endocrinology and metabolism. 106(7):1867-1881.
Abstract
Context Iodide transport defect (ITD) (Online Mendelian Inheritance in Man #274400) is an uncommon cause of dyshormonogenic congenital hypothyroidism due to loss-of-function variants in the SLC5A5 gene, which encodes the sodium/iodide symporter (NIS), causing deficient iodide accumulation in thyroid follicular cells.
Objective To determine the molecular basis of a patient´s ITD clinical phenotype.
Patient The propositus was diagnosed with dyshormonogenic congenital hypothyroidism with minimal 99mTc-pertechnetate accumulation in a eutopic thyroid gland.
Design Propositus SLC5A5 gene was sequenced. Functional in vitro characterization of the novel NIS variant was performed.
Results Sanger sequencing revealed a novel homozygous missense p.G561E NIS variant. Mechanistically, the G561E substitution reduces iodide uptake, because targeting of G561E NIS to the plasma membrane is reduced. Biochemical analyses revealed that G561E impairs the recognition of an adjacent tryptophan-acidic motif by the kinesin-1 subunit kinesin light chain 2 (KLC2), interfering with NIS maturation beyond the endoplasmic reticulum, and reducing iodide accumulation. Structural bioinformatic analysis suggests that G561E shifts the equilibrium of the unstructured tryptophan-acidic motif towards a more structured conformation unrecognizable to KLC2. Consistently, knockdown of Klc2 causes defective NIS maturation and consequently decreases iodide accumulation in rat thyroid cells. Morpholino knockdown of klc2 reduces thyroid hormone synthesis in zebrafish larvae leading to a hypothyroid state as revealed by expression profiling of key genes related to the hypothalamic-pituitary-thyroid axis.
Conclusions We report a novel NIS pathogenic variant associated with dyshormonogenic congenital hypothyroidism. Detailed molecular characterization of G561E NIS uncovered the significance of KLC2 in thyroid physiology.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping