PUBLICATION
An optimized method for adult zebrafish brain-tissue dissociation that allows access mitochondrial function under healthy and epileptic conditions
- Authors
- Moro, L., Rech, G., Linazzi, A.M., Dos Santos, T.G., de Oliveira, D.L.
- ID
- ZDB-PUB-210428-5
- Date
- 2021
- Source
- Brain research 1765: 147498 (Journal)
- Registered Authors
- Keywords
- adult zebrafish, bioenergetics, brain, epilepsy, mitochondria
- MeSH Terms
-
- Animals
- Brain/metabolism
- Brain/physiology
- Cell Respiration/physiology
- Disease Models, Animal
- Energy Metabolism/drug effects
- Epilepsy/metabolism
- Female
- Histocytological Preparation Techniques/methods*
- Kainic Acid/pharmacology
- Male
- Mitochondria/metabolism*
- Oxidative Phosphorylation
- Oxygen Consumption/physiology
- Pentylenetetrazole/pharmacology
- Tissue Preservation/methods*
- Zebrafish
- PubMed
- 33894225 Full text @ Brain Res.
Citation
Moro, L., Rech, G., Linazzi, A.M., Dos Santos, T.G., de Oliveira, D.L. (2021) An optimized method for adult zebrafish brain-tissue dissociation that allows access mitochondrial function under healthy and epileptic conditions. Brain research. 1765:147498.
Abstract
Mitochondria play key roles in brain metabolism. Not surprisingly, mitochondria dysfunction is a ubiquitous cause of neurodegenerative diseases. In turn, acquired forms of epilepsy etiology is specifically intriguing since mitochondria function and dysfunction remain not completely enlightened. Investigation in the field includes models of epileptic disorder using mainly rodents followed by mitochondrial variables evaluation, which in general evidenced controversial data. So, we considered the efforts and limitations in this research field and we took into account that sample preparation and quality are critical for bioenergetics investigation. For these reasons the aim of the present study was to develop a thorough protocol for adult zebrafish brain-tissue dissociation to evaluate oxygen consumption flux and reach the bioenergetics profile in health and models of epileptic disorder in both, in vitro using pentylenetetrazole (PTZ) and N-methyl-D-Aspartic acid (NMDA), and in vivo after kainic acid (KA)-induced status epilepticus. In conclusion, we verify that fire-polished glass Pasteur pipette is eligible to brain-tissue dissociation and to study mitochondrial function and dysfunction in adult zebrafish. The results give evidence for large effect size in increase of coupling efficiency respiration (p/O2) correlated to treatment with PTZ and spare respiratory capacity (SRC) in KA-induced model indicating oxidative phosphorylation (OXPHOS) variable alterations. Further investigation is needed in order to clarify the bioenergetics role as well as other mitochondrial functions in epilepsy.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping