Protocol for analysis of integrin-mediated cell adhesion of lateral plate mesoderm cells isolated from zebrafish embryos
- Rho, S.S., Oguri-Nakamura, E., Ando, K., Yamamoto, K., Takagi, Y., Fukuhara, S.
- STAR protocols 2: 100428 (Other)
- Registered Authors
- Ando, Koji, Fukuhara, Shigetomo
- Cell Biology, Cell culture, Cell isolation, Microscopy, Model Organisms
- MeSH Terms
- Cell Adhesion/physiology*
- Cells, Cultured
- Cytological Techniques/methods*
- Embryo, Nonmammalian/cytology*
- 33870229 Full text @ STAR Protoc
Rho, S.S., Oguri-Nakamura, E., Ando, K., Yamamoto, K., Takagi, Y., Fukuhara, S. (2021) Protocol for analysis of integrin-mediated cell adhesion of lateral plate mesoderm cells isolated from zebrafish embryos. STAR protocols. 2:100428.
Lateral plate mesoderm (LPM) cells differentiate into various cell types including endothelial and hematopoietic cells. In zebrafish embryos, LPM cells migrate toward the midline along the ventral surfaces of somites during which their cell fate specification depends upon efficient integrin-mediated cell adhesion and migration. Herein, we present a protocol for analysis of integrin-mediated cell adhesion of LPM cells isolated from zebrafish embryos. This allows the study of the molecular mechanisms underlying integrin activation required for LPM cell fate specification. For complete details on the use and execution of this protocol, please refer to Rho et al. (2019).
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes