PUBLICATION
The diversity in sensitivity of TRPA1 and TRPV1 of various animals to polyphenols
- Authors
- Takahashi, S., Kurogi, M., Saitoh, O.
- ID
- ZDB-PUB-210413-9
- Date
- 2021
- Source
- Biomedical research (Tokyo, Japan) 42: 43-51 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Snakes
- Oryzias
- Flavonoids/pharmacology
- TRPV Cation Channels/biosynthesis*
- HEK293 Cells
- Mice
- Neurons/drug effects*
- TRPA1 Cation Channel/biosynthesis*
- Animals
- Ambystoma mexicanum
- Chickens
- Calcium/metabolism
- Polyphenols/chemistry
- Polyphenols/pharmacology*
- Amphibians
- Rats
- Tannins/pharmacology
- Zebrafish
- Humans
- Catechin/analogs & derivatives
- Catechin/pharmacology
- Zebrafish Proteins/biosynthesis
- PubMed
- 33840685 Full text @ Biomed Res
Citation
Takahashi, S., Kurogi, M., Saitoh, O. (2021) The diversity in sensitivity of TRPA1 and TRPV1 of various animals to polyphenols. Biomedical research (Tokyo, Japan). 42:43-51.
Abstract
The perception of tastes is sensed by the receptors that stimulate sensory cells. We previously reported that TRPA1 and TRPV1 channels expressed in the oral cavity of mammals, are activated by the auto-oxidized product of epigallocatechin gallate (oxiEGCG), a major astringent catechin in green tea. Here, we investigated and compared the sensitivity of TRPA1 and TRPV1 from various animals to astringent polyphenols. We selected three polyphenols, oxiEGCG, tannic acid and myricetin. HEK293T cells expressing TRPA1 or TRPV1 from mammal, bird, reptile, amphibian, and fish, were analyzed for their activation by the Ca2+-imaging. We found the apparent diversity in the polyphenol-sensitivity among various animals. Mammalian TRPs showed relatively higher sensitivity to polyphenols, and especially, human TRPA1 and TRPV1 could be activated by all of three polyphenols at 20 μM. Reptile TRP channels, however, were insensitive to any polyphenols examined. Moreover, the polyphenol-sensitivity of zebrafish TRPA1 and TRPV1 was quite different from that of medaka TRP channels. Since many polyphenols are present in plants and the sensing of polyphenols using TRP channels in the oral cavity might cause astringent taste, the observed diversity of the polyphenol-sensitivity of TRP channels might be involved in the divergence in the food habit of various animals.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping