PUBLICATION
Cytokinetic abscission is part of the midblastula transition in early zebrafish embryogenesis
- Authors
- Adar-Levor, S., Nachmias, D., Gal-Oz, S.T., Jahn, Y.M., Peyrieras, N., Zaritsky, A., Birnbaum, R.Y., Elia, N.
- ID
- ZDB-PUB-210411-1
- Date
- 2021
- Source
- Proceedings of the National Academy of Sciences of the United States of America 118(15): (Journal)
- Registered Authors
- Birnbaum, Ramon
- Keywords
- MBT, abscission, cell division, development, microtubules
- MeSH Terms
-
- Cell Shape
- Animals
- Zebrafish Proteins/metabolism
- Endosomal Sorting Complexes Required for Transport/metabolism
- Zebrafish
- Cytokinesis*
- Blastula/cytology
- Blastula/embryology*
- Blastula/metabolism
- PubMed
- 33837152 Full text @ Proc. Natl. Acad. Sci. USA
Citation
Adar-Levor, S., Nachmias, D., Gal-Oz, S.T., Jahn, Y.M., Peyrieras, N., Zaritsky, A., Birnbaum, R.Y., Elia, N. (2021) Cytokinetic abscission is part of the midblastula transition in early zebrafish embryogenesis. Proceedings of the National Academy of Sciences of the United States of America. 118(15):.
Abstract
Animal cytokinesis ends with the formation of a thin intercellular membrane bridge that connects the two newly formed sibling cells, which is ultimately resolved by abscission. While mitosis is completed within 15 min, the intercellular bridge can persist for hours, maintaining a physical connection between sibling cells and allowing exchange of cytosolic components. Although cell-cell communication is fundamental for development, the role of intercellular bridges during embryogenesis has not been fully elucidated. In this work, we characterized the spatiotemporal characteristics of the intercellular bridge during early zebrafish development. We found that abscission is delayed during the rapid division cycles that occur in the early embryo, giving rise to the formation of interconnected cell clusters. Abscission was accelerated when the embryo entered the midblastula transition (MBT) phase. Components of the ESCRT machinery, which drives abscission, were enriched at intercellular bridges post-MBT and, interfering with ESCRT function, extended abscission beyond MBT. Hallmark features of MBT, including transcription onset and cell shape modulations, were more similar in interconnected sibling cells compared to other neighboring cells. Collectively, our findings suggest that delayed abscission in the early embryo allows clusters of cells to coordinate their behavior during embryonic development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping