PUBLICATION
Toxicity and Anti-Proliferative Properties of Anisomeles indica Ethanol Extract on Cervical Cancer HeLa Cells and Zebrafish Embryos
- Authors
- Bich-Loan, N.T., Kien, K.T., Thanh, N.L., Kim-Thanh, N.T., Huy, N.Q., The-Hai, P., Muller, M., Nachtergael, A., Duez, P., Thang, N.D.
- ID
- ZDB-PUB-210407-37
- Date
- 2021
- Source
- Life (Basel, Switzerland) 11(3): (Journal)
- Registered Authors
- Muller, Marc
- Keywords
- Anisomeles indica, HeLa cell, apoptosis, cell cycle arrest, colony formation, gene expression, medicinal plant, zebrafish
- MeSH Terms
- none
- PubMed
- 33804714 Full text @ Life (Basel)
Citation
Bich-Loan, N.T., Kien, K.T., Thanh, N.L., Kim-Thanh, N.T., Huy, N.Q., The-Hai, P., Muller, M., Nachtergael, A., Duez, P., Thang, N.D. (2021) Toxicity and Anti-Proliferative Properties of Anisomeles indica Ethanol Extract on Cervical Cancer HeLa Cells and Zebrafish Embryos. Life (Basel, Switzerland). 11(3):.
Abstract
In this study, we showed that crude extract of Anisomeles indica (AI-EtE) expressed its toxicity to HeLa cells with an IC50 dose of 38.8 µg/mL and to zebrafish embryos with malformations, lethality and hatching inhibition at 72-hpf at doses higher than 75 µg/mL. More interestingly, flow cytometry revealed that AI-EtE significantly promoted the number of cells entering apoptotic. Accordingly, the transcript levels of BAX, CASPASE-8, and CASPASE-3 in the cells treated with AI-EtE at IC50 dose were 1.55-, 1.62-, and 2.45-fold higher than those in the control cells, respectively. Moreover, treatment with AI-EtE caused cell cycle arrest at the G1 phase in a p53-independent manner. Particularly, percentages of AI-EtE-treated cells in G1, S, G2/M were, respectively 85%, 6.7% and 6.4%; while percentages of control cells in G1, S, G2/M were 64%, 15% and 19%, respectively. Consistent with cell cycle arrest, the expressions of CDKN1A and CDNK2A in AI-EtE-treated cells were up-regulated 1.9- and 1.64-fold, respectively. Significantly, treatment with AI-EtE also decreased anchorage-independent growth of HeLa cells. In conclusion, we suggest that Anisomeles indica can be considered as a medicinal plant with a possible use against cervical cancer cells; however, the used dose should be carefully monitored, especially when applying to pregnant women.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping