PUBLICATION
A protocol for simultaneous Ca2+ and morphology imaging of brain endothelial tip cells in larval zebrafish
- Authors
- Liu, T.T., Hou, H., Du, J.L.
- ID
- ZDB-PUB-210330-11
- Date
- 2021
- Source
- STAR protocols 2: 100388 (Journal)
- Registered Authors
- Keywords
- Cell biology, Microscopy, Model organisms, Neuroscience
- MeSH Terms
-
- Animals
- Brain/blood supply
- Brain/cytology
- Brain Mapping/methods*
- Calcium/metabolism*
- Diagnostic Imaging/methods
- Endothelial Cells/metabolism
- Fluorescent Antibody Technique/methods
- Larva/metabolism
- Neovascularization, Physiologic/physiology*
- Zebrafish/anatomy & histology
- Zebrafish/physiology
- PubMed
- 33778782 Full text @ STAR Protoc
Citation
Liu, T.T., Hou, H., Du, J.L. (2021) A protocol for simultaneous Ca2+ and morphology imaging of brain endothelial tip cells in larval zebrafish. STAR protocols. 2:100388.
Abstract
Endothelial tip cells (ETCs) located at growing blood vessels display high morphological dynamics and associated intracellular Ca2+ activities with different spatiotemporal patterns during migration. Examining the Ca2+ activity and morphological dynamics of ETCs will provide an insight for understanding the mechanism of vascular development in organs, including the brain. Here, we describe a method for simultaneous monitoring and relevant analysis of the Ca2+ activity and morphology of growing brain ETCs in larval zebrafish. For complete details on the use and execution of this protocol, please refer to Liu et al. (2020).
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping